3gj4

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[[Image:3gj4.png|left|200px]]
 
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{{STRUCTURE_3gj4| PDB=3gj4 | SCENE= }}
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==Crystal structure of human RanGDP-Nup153ZnF3 complex==
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<StructureSection load='3gj4' size='340' side='right'caption='[[3gj4]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3gj4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GJ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gj4 OCA], [https://pdbe.org/3gj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gj4 RCSB], [https://www.ebi.ac.uk/pdbsum/3gj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gj4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RAN_HUMAN RAN_HUMAN] GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref> Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.<ref>PMID:10400640</ref> <ref>PMID:8692944</ref> <ref>PMID:18591255</ref> <ref>PMID:18617507</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gj/3gj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3gj4 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The nuclear pore complex (NPC) resides in circular openings within the nuclear envelope and serves as the sole conduit to facilitate nucleocytoplasmic transport in eukaryotes. The asymmetric distribution of the small G protein Ran across the nuclear envelope regulates directionality of protein transport. Ran interacts with the NPC of metazoa via two asymmetrically localized components, Nup153 at the nuclear face and Nup358 at the cytoplasmic face. Both nucleoporins contain a stretch of distinct, Ran-binding zinc finger domains. Here, we present six crystal structures of Nup153-zinc fingers in complex with Ran and a 1.48 A crystal structure of RanGDP. Crystal engineering allowed us to obtain well diffracting crystals so that all ZnF-Ran complex structures are refined to high resolution. Each of the four zinc finger modules of Nup153 binds one Ran molecule in apparently non-allosteric fashion. The affinity is measurably higher for RanGDP than for RanGTP and varies modestly between the individual zinc fingers. By microcalorimetric and mutational analysis, we determined that one specific hydrogen bond accounts for most of the differences in the binding affinity of individual zinc fingers. Genomic analysis reveals that only in animals do NPCs contain Ran-binding zinc fingers. We speculate that these organisms evolved a mechanism to maintain a high local concentration of Ran at the vicinity of the NPC, using this zinc finger domain as a sink.
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===Crystal structure of human RanGDP-Nup153ZnF3 complex===
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Crystallographic and biochemical analysis of the Ran-binding zinc finger domain.,Partridge JR, Schwartz TU J Mol Biol. 2009 Aug 14;391(2):375-89. Epub 2009 Jun 6. PMID:19505478<ref>PMID:19505478</ref>
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{{ABSTRACT_PUBMED_19505478}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3gj4" style="background-color:#fffaf0;"></div>
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[[3gj4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GJ4 OCA].
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==See Also==
==See Also==
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*[[Nucleoporin|Nucleoporin]]
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*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
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*[[Nucleoporin 3D structures|Nucleoporin 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:019505478</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Partridge, J R.]]
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[[Category: Partridge JR]]
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[[Category: Schwartz, T U.]]
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[[Category: Schwartz TU]]
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[[Category: Dna-binding]]
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[[Category: G protein]]
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[[Category: Gdp]]
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[[Category: Gtp-binding]]
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[[Category: Host-virus interaction]]
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[[Category: Isopeptide bond]]
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[[Category: Metal-binding]]
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[[Category: Mrna transport]]
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[[Category: Nuclear pore]]
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[[Category: Nuclear pore complex]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleus]]
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[[Category: Nup153]]
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[[Category: Phosphoprotein]]
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[[Category: Protein transport]]
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[[Category: Ran]]
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[[Category: Translocation]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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[[Category: Zinc finger]]
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[[Category: Zinc-finger]]
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Current revision

Crystal structure of human RanGDP-Nup153ZnF3 complex

PDB ID 3gj4

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