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1n7z

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[[Image:1n7z.jpg|left|200px]]<br /><applet load="1n7z" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n7z, resolution 2.00&Aring;" />
 
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'''Structure and location of gene product 8 in the bacteriophage T4 baseplate'''<br />
 
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==Overview==
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==Structure and location of gene product 8 in the bacteriophage T4 baseplate==
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Many bacteriophages, such as T4, T7, RB49, and phi29, have complex, sometimes multilayered, tails that facilitate an almost 100% success rate for the viral particles to infect host cells. In bacteriophage T4, there is a baseplate, which is a multiprotein assembly, at the distal end of the contractile tail. The baseplate communicates to the tail that the phage fibers have attached to the host cell, thereby initiating the infection process. Gene product 8 (gp8), whose amino acid sequence consists of 334 residues, is one of at least 16 different structural proteins that constitute the T4 baseplate and is the sixth baseplate protein whose structure has been determined. A 2.0A resolution X-ray structure of gp8 shows that the two-domain protein forms a dimer, in which each monomer consists of a three-layered beta-sandwich with two loops, each containing an alpha-helix at the opposite sides of the sandwich. The crystals of gp8 were produced in the presence of concentrated chloride and bromide ions, resulting in at least 11 halide-binding sites per monomer. Five halide sites, situated at the N termini of alpha-helices, have a protein environment observed in other halide-containing protein crystal structures. The computer programs EMfit and SITUS were used to determine the positions of six gp8 dimers within the 12A resolution cryo-electron microscopy image reconstruction of the baseplate-tail tube complex. The gp8 dimers were found to be located in the upper part of the baseplate outer rim. About 20% of the gp8 surface is involved in contacts with other baseplate proteins, presumed to be gp6, gp7, and gp10. With the structure determination of gp8, a total of 53% of the volume of the baseplate has now been interpreted in terms of its atomic structure.
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<StructureSection load='1n7z' size='340' side='right'caption='[[1n7z]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1n7z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N7Z FirstGlance]. <br>
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1N7Z is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N7Z OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n7z OCA], [https://pdbe.org/1n7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n7z RCSB], [https://www.ebi.ac.uk/pdbsum/1n7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n7z ProSAT]</span></td></tr>
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Structure and location of gene product 8 in the bacteriophage T4 baseplate., Leiman PG, Shneider MM, Kostyuchenko VA, Chipman PR, Mesyanzhinov VV, Rossmann MG, J Mol Biol. 2003 May 9;328(4):821-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12729757 12729757]
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</table>
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[[Category: Bacteriophage t4]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/BP08_BPT4 BP08_BPT4] Baseplate protein that is part of the baseplate wedge. Involved in the tail assembly.<ref>PMID:15315755</ref> <ref>PMID:21129200</ref>
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[[Category: Chipman, P R.]]
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== Evolutionary Conservation ==
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[[Category: Kostyuchenko, V A.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Leiman, P G.]]
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Check<jmol>
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[[Category: Mesyanzhinov, V V.]]
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<jmolCheckbox>
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[[Category: Rossmann, M G.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n7/1n7z_consurf.spt"</scriptWhenChecked>
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[[Category: Shneider, M M.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: CL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: bacteriophage t4]]
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</jmolCheckbox>
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[[Category: baseplate]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n7z ConSurf].
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[[Category: beta sandwich]]
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<div style="clear:both"></div>
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[[Category: dimer]]
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== References ==
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<references/>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:20 2008''
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__TOC__
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</StructureSection>
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[[Category: Escherichia virus T4]]
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[[Category: Large Structures]]
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[[Category: Chipman PR]]
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[[Category: Kostyuchenko VA]]
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[[Category: Leiman PG]]
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[[Category: Mesyanzhinov VV]]
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[[Category: Rossmann MG]]
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[[Category: Shneider MM]]

Current revision

Structure and location of gene product 8 in the bacteriophage T4 baseplate

PDB ID 1n7z

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