1n8w

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[[Image:1n8w.gif|left|200px]]<br /><applet load="1n8w" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n8w, resolution 2.70&Aring;" />
 
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'''Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis'''<br />
 
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==Overview==
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==Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis==
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Establishment or maintenance of a persistent infection by Mycobacterium tuberculosis requires the glyoxylate pathway. This is a bypass of the tricarboxylic acid cycle in which isocitrate lyase and malate synthase (GlcB) catalyze the net incorporation of carbon during growth of microorganisms on acetate or fatty acids as the primary carbon source. The glcB gene from M. tuberculosis, which encodes malate synthase, was cloned, and GlcB was expressed in Escherichia coli. The influence of media conditions on expression in M. tuberculosis indicated that this enzyme is regulated differentially to isocitrate lyase. Purified GlcB had K(m) values of 57 and 30 microm for its substrates glyoxylate and acetyl coenzyme A, respectively, and was inhibited by bromopyruvate, oxalate, and phosphoenolpyruvate. The GlcB structure was solved to 2.1-A resolution in the presence of glyoxylate and magnesium. We also report the structure of GlcB in complex with the products of the reaction, coenzyme A and malate, solved to 2.7-A resolution. Coenzyme A binds in a bent conformation, and the details of its interactions are described, together with implications on the enzyme mechanism.
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<StructureSection load='1n8w' size='340' side='right'caption='[[1n8w]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n8w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8W FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=GLV:GLYOXYLIC+ACID'>GLV</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8w OCA], [https://pdbe.org/1n8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8w RCSB], [https://www.ebi.ac.uk/pdbsum/1n8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8w ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MASZ_MYCTU MASZ_MYCTU] Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA (By similarity).[HAMAP-Rule:MF_00641]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8w ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1N8W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MLT:'>MLT</scene>, <scene name='pdbligand=COA:'>COA</scene> and <scene name='pdbligand=GLV:'>GLV</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Malate_synthase Malate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.9 2.3.3.9] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8W OCA].
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*[[Malate synthase 3D structures|Malate synthase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis., Smith CV, Huang CC, Miczak A, Russell DG, Sacchettini JC, Honer zu Bentrup K, J Biol Chem. 2003 Jan 17;278(3):1735-43. Epub 2002 Oct 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12393860 12393860]
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[[Category: Large Structures]]
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[[Category: Malate synthase]]
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[[Category: Mycobacterium tuberculosis]]
[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Honer zu Bentrup K]]
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[[Category: Bentrup, K Honer zu.]]
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[[Category: Huang CC]]
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[[Category: Huang, C C.]]
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[[Category: Miczak A]]
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[[Category: Miczak, A.]]
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[[Category: Russell DG]]
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[[Category: Russell, D G.]]
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[[Category: Sacchettini JC]]
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[[Category: Sacchettini, J C.]]
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[[Category: Smith CV]]
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[[Category: Smith, C V.]]
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[[Category: TBSGC, TB Structural Genomics Consortium.]]
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[[Category: COA]]
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[[Category: GLV]]
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[[Category: MG]]
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[[Category: MLT]]
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[[Category: SO4]]
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[[Category: coenzyme a]]
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[[Category: glcb]]
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[[Category: glyoxylate]]
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[[Category: glyoxylate pathway]]
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[[Category: malate]]
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[[Category: malate synthase]]
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[[Category: mycobacterium tuberculosis]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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[[Category: tb structural genomics consortium]]
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[[Category: tbsgc]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:29 2008''
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Current revision

Biochemical and Structural Studies of Malate Synthase from Mycobacterium tuberculosis

PDB ID 1n8w

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