1n8v

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[[Image:1n8v.gif|left|200px]]<br /><applet load="1n8v" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1n8v, resolution 1.39&Aring;" />
 
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'''Chemosensory Protein in complex with bromo-dodecanol'''<br />
 
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==Overview==
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==Chemosensory Protein in complex with bromo-dodecanol==
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Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of insect species. The x-ray structure of CSPMbraA6, a 112-aa antennal protein from the moth Mamestra brassicae (Mbra), was shown to exhibit a novel type of alpha-helical fold. We have performed a structural and binding study of CSPMbraA6 to get some insights into its possible molecular function. Tryptophan fluorescence quenching demonstrates the ability of CSPMbraA6 to bind several types of semio-chemicals or surrogate ligands with microM K(d). Its crystal structure in complex with one of these compounds, 12-bromo-dodecanol, reveals extensive conformational changes on binding, resulting in the formation of a large cavity filled by three ligand molecules. Furthermore, binding cooperativity was demonstrated for some ligands, suggesting a stepwise binding. The peculiar rearrangement of CSPMbraA6 conformation and the cooperativity phenomenon might trigger the recognition of chemicals by receptors and induce subsequent signal transduction.
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<StructureSection load='1n8v' size='340' side='right'caption='[[1n8v]], [[Resolution|resolution]] 1.39&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1n8v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8V FirstGlance]. <br>
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1N8V is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mamestra_brassicae Mamestra brassicae] with <scene name='pdbligand=BDD:'>BDD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8V OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.39&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BDD:BROMO-DODECANOL'>BDD</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8v OCA], [https://pdbe.org/1n8v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8v RCSB], [https://www.ebi.ac.uk/pdbsum/1n8v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8v ProSAT]</span></td></tr>
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Moth chemosensory protein exhibits drastic conformational changes and cooperativity on ligand binding., Campanacci V, Lartigue A, Hallberg BM, Jones TA, Giudici-Orticoni MT, Tegoni M, Cambillau C, Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5069-74. Epub 2003 Apr 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12697900 12697900]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9NG96_MAMBR Q9NG96_MAMBR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8v_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8v ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mamestra brassicae]]
[[Category: Mamestra brassicae]]
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[[Category: Single protein]]
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[[Category: Cambillau C]]
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[[Category: Cambillau, C.]]
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[[Category: Campanacci V]]
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[[Category: Campanacci, V.]]
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[[Category: Giudici-Orticoni MT]]
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[[Category: Giudici-Orticoni, M T]]
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[[Category: Hallberg BM]]
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[[Category: Hallberg, B M.]]
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[[Category: Jones TA]]
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[[Category: Jones, T A.]]
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[[Category: Lartigue A]]
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[[Category: Lartigue, A.]]
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[[Category: Tegoni M]]
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[[Category: Tegoni, M.]]
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[[Category: BDD]]
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[[Category: lipid binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:31 2008''
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Current revision

Chemosensory Protein in complex with bromo-dodecanol

PDB ID 1n8v

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