1na0

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[[Image:1na0.jpg|left|200px]]<br /><applet load="1na0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1na0, resolution 1.60&Aring;" />
 
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'''Design of Stable alpha-Helical Arrays from an Idealized TPR Motif'''<br />
 
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==Overview==
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==Design of Stable alpha-Helical Arrays from an Idealized TPR Motif==
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The tetratricopeptide repeat (TPR) is a 34-amino acid alpha-helical motif that occurs in over 300 different proteins. In the different proteins, three to sixteen or more TPR motifs occur in tandem arrays and function to mediate protein-protein interactions. The binding specificity of each TPR protein is different, although the underlying structural motif is the same. Here we describe a statistical approach to the design of an idealized TPR motif. We present the high-resolution X-ray crystal structures (to 1.55 and 1.6 A) of designed TPR proteins and describe their solution properties and stability. A detailed analysis of these structures provides an understanding of the TPR motif, how it is repeated to give helical arrays with different superhelical twists, and how a very stable framework may be constructed for future functional designs.
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<StructureSection load='1na0' size='340' side='right'caption='[[1na0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1na0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unidentified Unidentified]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NA0 FirstGlance]. <br>
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1NA0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=PB:'>PB</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=NA:'>NA</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=IPT:'>IPT</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NA0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=IPT:ISOPROPYL-1-BETA-D-THIOGALACTOSIDE'>IPT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PB:LEAD+(II)+ION'>PB</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1na0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1na0 OCA], [https://pdbe.org/1na0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1na0 RCSB], [https://www.ebi.ac.uk/pdbsum/1na0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1na0 ProSAT]</span></td></tr>
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Design of stable alpha-helical arrays from an idealized TPR motif., Main ER, Xiong Y, Cocco MJ, D'Andrea L, Regan L, Structure. 2003 May;11(5):497-508. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737816 12737816]
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</table>
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[[Category: ]]
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== Evolutionary Conservation ==
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[[Category: Protein complex]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Andrea, L D.]]
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Check<jmol>
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[[Category: Cocco, M.]]
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<jmolCheckbox>
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[[Category: Main, E.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1na0_consurf.spt"</scriptWhenChecked>
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[[Category: Regan, L.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Xiong, Y.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: ACT]]
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</jmolCheckbox>
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[[Category: CL]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1na0 ConSurf].
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[[Category: IPT]]
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<div style="clear:both"></div>
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[[Category: MG]]
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__TOC__
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[[Category: NA]]
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</StructureSection>
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[[Category: PB]]
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[[Category: Large Structures]]
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[[Category: design]]
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[[Category: Unidentified]]
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[[Category: tpr]]
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[[Category: Cocco M]]
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[[Category: D'Andrea L]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:03:50 2008''
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[[Category: Main E]]
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[[Category: Regan L]]
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[[Category: Xiong Y]]

Current revision

Design of Stable alpha-Helical Arrays from an Idealized TPR Motif

PDB ID 1na0

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