1a5o

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[[Image:1a5o.gif|left|200px]]<br />
 
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<applet load="1a5o" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1a5o, resolution 2.5&Aring;" />
 
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'''K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL'''<br />
 
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==Overview==
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==K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL==
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Klebsiella aerogenes urease possesses a dinuclear metallocenter in which, two nickel atoms are bridged by carbamylated Lys217. To assess whether, carbamate-specific chemistry is required for urease activity, site-directed mutagenesis and chemical rescue strategies were combined in, efforts to place a carboxylate group at the location of this metal ligand., Urease variants with Lys217 replaced by Glu, Cys, and Ala (K217E, K217C/C319A, and K217A proteins) were purified, shown to be activated by, incubation with small organic acids plus Ni(II), and structurally, characterized. K217C/C319A urease possessed a second change in which, Cys319 was replaced by Ala in order to facilitate efforts to chemically, modify Cys217; however, this covalent modification approach did not, produce active ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9558361 (full description)]]
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<StructureSection load='1a5o' size='340' side='right'caption='[[1a5o]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1a5o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5o OCA], [https://pdbe.org/1a5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5o RCSB], [https://www.ebi.ac.uk/pdbsum/1a5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/URE3_KLEAE URE3_KLEAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5o_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5o ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1A5O is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Klebsiella_aerogenes Klebsiella aerogenes]] with NI and FMT as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Urease Urease]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.5 3.5.1.5]]. Structure known Active Sites: ACT and NIL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1A5O OCA]].
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*[[Urease 3D structures|Urease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Chemical rescue of Klebsiella aerogenes urease variants lacking the carbamylated-lysine nickel ligand., Pearson MA, Schaller RA, Michel LO, Karplus PA, Hausinger RP, Biochemistry. 1998 Apr 28;37(17):6214-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9558361 9558361]
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[[Category: Klebsiella aerogenes]]
[[Category: Klebsiella aerogenes]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Urease]]
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[[Category: Hausinger RP]]
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[[Category: Hausinger, R.P.]]
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[[Category: Karplus PA]]
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[[Category: Karplus, P.A.]]
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[[Category: Michel LO]]
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[[Category: Michel, L.O.]]
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[[Category: Pearson MA]]
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[[Category: Pearson, M.A.]]
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[[Category: Schaller RA]]
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[[Category: Schaller, R.A.]]
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[[Category: FMT]]
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[[Category: NI]]
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[[Category: hydrolase (urea amido)]]
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[[Category: mutant]]
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[[Category: nickel metalloenzyme]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:45:24 2007''
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Current revision

K217C VARIANT OF KLEBSIELLA AEROGENES UREASE, CHEMICALLY RESCUED BY FORMATE AND NICKEL

PDB ID 1a5o

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