2q69

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[[Image:2q69.png|left|200px]]
 
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{{STRUCTURE_2q69| PDB=2q69 | SCENE= }}
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==Crystal Structure of Nak channel D66N mutant==
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<StructureSection load='2q69' size='340' side='right'caption='[[2q69]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2q69]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q69 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q69 OCA], [https://pdbe.org/2q69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q69 RCSB], [https://www.ebi.ac.uk/pdbsum/2q69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q69 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q81HW2_BACCR Q81HW2_BACCR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/2q69_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q69 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Apparent blockage of monovalent cation currents by the permeating blocker Ca(2+) is a physiologically essential phenomenon relevant to cyclic nucleotide-gated (CNG) channels. The recently determined crystal structure of a bacterial homolog of CNG channel pores, the NaK channel, revealed a Ca(2+) binding site at the extracellular entrance to the selectivity filter. This site is not formed by the side-chain carboxylate groups from the conserved acidic residue, Asp-66 in NaK, conventionally thought to directly chelate Ca(2+) in CNG channels, but rather by the backbone carbonyl groups of residue Gly-67. Here we present a detailed structural analysis of the NaK channel with a focus on Ca(2+) permeability and blockage. Our results confirm that the Asp-66 residue, although not involved in direct chelation of Ca(2+), plays an essential role in external Ca(2+) binding. Furthermore, we give evidence for the presence of a second Ca(2+) binding site within the NaK selectivity filter where monovalent cations also bind, providing a structural basis for Ca(2+) permeation through the NaK pore. Compared with other Ca(2+)-binding proteins, both sites in NaK present a novel mode of Ca(2+) chelation, using only backbone carbonyl oxygen atoms from residues in the selectivity filter. The external site is under indirect control by an acidic residue (Asp-66), making it Ca(2+)-specific. These findings give us a glimpse of the possible underlying mechanisms allowing Ca(2+) to act both as a permeating ion and blocker of CNG channels and raise the possibility of a similar chemistry governing Ca(2+) chelation in Ca(2+) channels.
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===Crystal Structure of Nak channel D66N mutant===
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Structural insight into Ca2+ specificity in tetrameric cation channels.,Alam A, Shi N, Jiang Y Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15334-9. Epub 2007 Sep 18. PMID:17878296<ref>PMID:17878296</ref>
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{{ABSTRACT_PUBMED_17878296}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2q69" style="background-color:#fffaf0;"></div>
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[[2q69]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_cereus Bacillus cereus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q69 OCA].
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==See Also==
==See Also==
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*[[Potassium Channel|Potassium Channel]]
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*[[Potassium channel 3D structures|Potassium channel 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:017878296</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Bacillus cereus]]
[[Category: Bacillus cereus]]
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[[Category: Alam, A.]]
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[[Category: Large Structures]]
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[[Category: Jiang, Y.]]
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[[Category: Alam A]]
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[[Category: Shi, N.]]
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[[Category: Jiang Y]]
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[[Category: Central cavity]]
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[[Category: Shi N]]
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[[Category: Helix bundle]]
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[[Category: Inverted teepee]]
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[[Category: Ion binding]]
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[[Category: Membrane protein]]
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[[Category: Metal transport]]
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[[Category: Tetramer]]
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Current revision

Crystal Structure of Nak channel D66N mutant

PDB ID 2q69

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