3k4g

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[[Image:3k4g.png|left|200px]]
 
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{{STRUCTURE_3k4g| PDB=3k4g | SCENE= }}
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==Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain==
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<StructureSection load='3k4g' size='340' side='right'caption='[[3k4g]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3k4g]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K4G FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k4g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k4g OCA], [https://pdbe.org/3k4g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k4g RCSB], [https://www.ebi.ac.uk/pdbsum/3k4g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k4g ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOA_ECOLI RPOA_ECOLI] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/3k4g_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3k4g ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The alpha subunit C-terminal domain (alphaCTD) of RNA polymerase (RNAP) is a key element in transcription activation in Escherichia coli, possessing determinants responsible for the interaction of RNAP with DNA and with transcription factors. Here, the crystal structure of E. coli alphaCTD (alpha subunit residues 245-329) determined to 2.0 A resolution is reported. Crystals were obtained after reductive methylation of the recombinantly expressed domain. The crystals belonged to space group P2(1) and possessed both pseudo-translational symmetry and pseudo-merohedral twinning. The refined coordinate model (R factor = 0.193, R(free) = 0.236) has improved geometry compared with prior lower resolution determinations of the alphaCTD structure [Jeon et al. (1995), Science, 270, 1495-1497; Benoff et al. (2002), Science, 297, 1562-1566]. An extensive dimerization interface formed primarily by N- and C-terminal residues is also observed. The new coordinates will facilitate the improved modeling of alphaCTD-containing multi-component complexes visualized at lower resolution using X-ray crystallography and electron-microscopy reconstruction.
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===Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain===
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Structure of the Escherichia coli RNA polymerase alpha subunit C-terminal domain.,Lara-Gonzalez S, Birktoft JJ, Lawson CL Acta Crystallogr D Biol Crystallogr. 2010 Jul;66(Pt 7):806-12. Epub 2010, Jun 19. PMID:20606261<ref>PMID:20606261</ref>
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{{ABSTRACT_PUBMED_20606261}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3k4g" style="background-color:#fffaf0;"></div>
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[[3k4g]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K4G OCA].
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==See Also==
==See Also==
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*[[RNA polymerase|RNA polymerase]]
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020606261</ref><references group="xtra"/>
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__TOC__
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[[Category: DNA-directed RNA polymerase]]
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</StructureSection>
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[[Category: Escherichia coli k-12]]
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[[Category: Escherichia coli K-12]]
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[[Category: Birktoft, J.]]
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[[Category: Large Structures]]
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[[Category: Lara-Gonzalez, S.]]
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[[Category: Birktoft J]]
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[[Category: Lawson, C L.]]
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[[Category: Lara-Gonzalez S]]
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[[Category: Bacterial transcription regulation]]
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[[Category: Lawson CL]]
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[[Category: Dna-directed rna polymerase]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Transcription]]
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[[Category: Transferase]]
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Current revision

Crystal structure of E. coli RNA polymerase alpha subunit C-terminal domain

PDB ID 3k4g

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