1nj3

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[[Image:1nj3.gif|left|200px]]<br /><applet load="1nj3" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nj3" />
 
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'''Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4'''<br />
 
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==Overview==
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==Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4==
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Ubiquitylated proteins are directed into a large number of different cellular pathways through interactions with effector proteins that contain conserved ubiquitin binding motifs. Here, we report the solution structure and ubiquitin binding properties of one such motif, the Npl4 zinc finger or RanBP2/Nup358 zinc finger (NZF) domain. Npl4 NZF forms a compact module composed of four antiparallel beta-strands linked by three ordered loops. A single zinc ion is coordinated by four conserved cysteines from the first and third loops, which form two rubredoxin knuckles. Npl4 NZF binds specifically, but weakly, to free ubiquitin using a conserved 13TF14 dipeptide to interact with the "Ile-44" surface of ubiquitin. Our studies reveal the structure of this versatile class of protein binding domains and provide a means for identifying the subset of NZF domains likely to bind ubiquitin.
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<StructureSection load='1nj3' size='340' side='right'caption='[[1nj3]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nj3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NJ3 FirstGlance]. <br>
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1NJ3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJ3 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nj3 OCA], [https://pdbe.org/1nj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nj3 RCSB], [https://www.ebi.ac.uk/pdbsum/1nj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nj3 ProSAT]</span></td></tr>
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Structure and ubiquitin interactions of the conserved zinc finger domain of Npl4., Wang B, Alam SL, Meyer HH, Payne M, Stemmler TL, Davis DR, Sundquist WI, J Biol Chem. 2003 May 30;278(22):20225-34. Epub 2003 Mar 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12644454 12644454]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NPL4_RAT NPL4_RAT] The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope.<ref>PMID:10811609</ref> <ref>PMID:11781570</ref> <ref>PMID:11740563</ref> <ref>PMID:14636562</ref> <ref>PMID:12411482</ref> <ref>PMID:12644454</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nj/1nj3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nj3 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Alam SL]]
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[[Category: Alam, S L.]]
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[[Category: Davis DR]]
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[[Category: Davis, D R.]]
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[[Category: Meyer HH]]
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[[Category: Meyer, H H.]]
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[[Category: Payne M]]
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[[Category: Payne, M.]]
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[[Category: Stemmler TL]]
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[[Category: Stemmler, T L.]]
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[[Category: Sundquist WI]]
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[[Category: Sundquist, W I.]]
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[[Category: Wang B]]
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[[Category: Wang, B.]]
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[[Category: ZN]]
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[[Category: beta-ribbon]]
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[[Category: npl4]]
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[[Category: nzf domain]]
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[[Category: rubredoxin knuckle]]
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[[Category: ubiquitin]]
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[[Category: zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:06:37 2008''
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Current revision

Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4

PDB ID 1nj3

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