2is3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:13, 30 October 2024) (edit) (undo)
 
(5 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2is3.png|left|200px]]
 
-
{{STRUCTURE_2is3| PDB=2is3 | SCENE= }}
+
==Crystal Structure of Escherichia coli RNase T==
 +
<StructureSection load='2is3' size='340' side='right'caption='[[2is3]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2is3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IS3 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2is3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2is3 OCA], [https://pdbe.org/2is3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2is3 RCSB], [https://www.ebi.ac.uk/pdbsum/2is3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2is3 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RNT_ECOLI RNT_ECOLI] Responsible for the end-turnover of tRNA: specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis, especially in strains lacking other exoribonucleases.[HAMAP-Rule:MF_00157]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/is/2is3_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2is3 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The 3' processing of most bacterial precursor tRNAs involves exonucleolytic trimming to yield a mature CCA end. This step is carried out by RNase T, a member of the large DEDD family of exonucleases. We report the crystal structures of RNase T from Escherichia coli and Pseudomonas aeruginosa, which show that this enzyme adopts an opposing dimeric arrangement, with the catalytic DEDD residues from one monomer closely juxtaposed with a large basic patch on the other monomer. This arrangement suggests that RNase T has to be dimeric for substrate specificity, and agrees very well with prior site-directed mutagenesis studies. The dimeric architecture of RNase T is very similar to the arrangement seen in oligoribonuclease, another bacterial DEDD family exoribonuclease. The catalytic residues in these two enzymes are organized very similarly to the catalytic domain of the third DEDD family exoribonuclease in E. coli, RNase D, which is monomeric.
-
===Crystal Structure of Escherichia coli RNase T===
+
Crystal structure of RNase T, an exoribonuclease involved in tRNA maturation and end turnover.,Zuo Y, Zheng H, Wang Y, Chruszcz M, Cymborowski M, Skarina T, Savchenko A, Malhotra A, Minor W Structure. 2007 Apr;15(4):417-28. PMID:17437714<ref>PMID:17437714</ref>
-
{{ABSTRACT_PUBMED_17437714}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 2is3" style="background-color:#fffaf0;"></div>
-
[[2is3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IS3 OCA].
+
==See Also==
==See Also==
-
*[[Ribonuclease|Ribonuclease]]
+
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:017437714</ref><references group="xtra"/>
+
__TOC__
-
[[Category: Escherichia coli k-12]]
+
</StructureSection>
-
[[Category: Malhotra, A.]]
+
[[Category: Escherichia coli K-12]]
-
[[Category: Wang, Y.]]
+
[[Category: Large Structures]]
-
[[Category: Zuo, Y.]]
+
[[Category: Malhotra A]]
-
[[Category: Exonuclease]]
+
[[Category: Wang Y]]
-
[[Category: Exoribonuclease]]
+
[[Category: Zuo Y]]
-
[[Category: Hydrolase]]
+
-
[[Category: Nuclease]]
+
-
[[Category: Ribonuclease]]
+
-
[[Category: Rnase]]
+
-
[[Category: Stable rna maturation]]
+
-
[[Category: Trna end-turnover]]
+

Current revision

Crystal Structure of Escherichia coli RNase T

PDB ID 2is3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2is3"
Personal tools