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3lva

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[[Image:3lva.png|left|200px]]
 
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{{STRUCTURE_3lva| PDB=3lva | SCENE= }}
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==Crystal structure of colorless GFP-like protein from Aequorea coerulescens==
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<StructureSection load='3lva' size='340' side='right'caption='[[3lva]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3lva]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_coerulescens Aequorea coerulescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3LVA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GYS:[(4Z)-2-(1-AMINO-2-HYDROXYETHYL)-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYS</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3lva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lva OCA], [https://pdbe.org/3lva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3lva RCSB], [https://www.ebi.ac.uk/pdbsum/3lva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3lva ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6YGZ0_9CNID Q6YGZ0_9CNID]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/3lva_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3lva ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The acGFPL is the first-identified member of a novel, colorless and non-fluorescent group of green fluorescent protein (GFP)-like proteins. Its mutant aceGFP, with Gly replacing the invariant catalytic Glu-222, demonstrates a relatively fast maturation rate and bright green fluorescence (lambda(ex) = 480 nm, lambda(em) = 505 nm). The reverse G222E single mutation in aceGFP results in the immature, colorless variant aceGFP-G222E, which undergoes irreversible photoconversion to a green fluorescent state under UV light exposure. Here we present a high resolution crystallographic study of aceGFP and aceGFP-G222E in the immature and UV-photoconverted states. A unique and striking feature of the colorless aceGFP-G222E structure is the chromophore in the trapped intermediate state, where cyclization of the protein backbone has occurred, but Tyr-66 still stays in the native, non-oxidized form, with C(alpha) and C(beta) atoms in the sp(3) hybridization. This experimentally observed immature aceGFP-G222E structure, characterized by the non-coplanar arrangement of the imidazolone and phenolic rings, has been attributed to one of the intermediate states in the GFP chromophore biosynthesis. The UV irradiation (lambda = 250-300 nm) of aceGFP-G222E drives the chromophore maturation further to a green fluorescent state, characterized by the conventional coplanar bicyclic structure with the oxidized double Tyr-66 C(alpha)=C(beta) bond and the conjugated system of pi-electrons. Structure-based site-directed mutagenesis has revealed a critical role of the proximal Tyr-220 in the observed effects. In particular, an alternative reaction pathway via Tyr-220 rather than conventional wild type Glu-222 has been proposed for aceGFP maturation.
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===Crystal structure of colorless GFP-like protein from Aequorea coerulescens===
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Structural evidence for a dehydrated intermediate in green fluorescent protein chromophore biosynthesis.,Pletneva NV, Pletnev VZ, Lukyanov KA, Gurskaya NG, Goryacheva EA, Martynov VI, Wlodawer A, Dauter Z, Pletnev S J Biol Chem. 2010 May 21;285(21):15978-84. Epub 2010 Mar 9. PMID:20220148<ref>PMID:20220148</ref>
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{{ABSTRACT_PUBMED_20220148}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3lva" style="background-color:#fffaf0;"></div>
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[[3lva]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Aequorea_coerulescens Aequorea coerulescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LVA OCA].
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==See Also==
==See Also==
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*[[Alyssa Marsico/Sandbox 1|Alyssa Marsico/Sandbox 1]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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*[[Devon McCarthy/Sandbox 1|Devon McCarthy/Sandbox 1]]
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== References ==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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<references/>
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*[[Sandbox104|Sandbox104]]
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__TOC__
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*[[User:Joanne Lau/Sandbox 5|User:Joanne Lau/Sandbox 5]]
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</StructureSection>
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==Reference==
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<ref group="xtra">PMID:020220148</ref><references group="xtra"/>
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[[Category: Aequorea coerulescens]]
[[Category: Aequorea coerulescens]]
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[[Category: Pletnev, S V.]]
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[[Category: Large Structures]]
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[[Category: Pletnev, V Z.]]
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[[Category: Pletnev SV]]
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[[Category: Pletneva, N V.]]
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[[Category: Pletnev VZ]]
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[[Category: Chromophore biosynthesis]]
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[[Category: Pletneva NV]]
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[[Category: Fluorescent protein]]
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Current revision

Crystal structure of colorless GFP-like protein from Aequorea coerulescens

PDB ID 3lva

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