1nks

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:57, 14 February 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1nks.gif|left|200px]]<br /><applet load="1nks" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1nks, resolution 2.57&Aring;" />
 
-
'''ADENYLATE KINASE FROM SULFOLOBUS ACIDOCALDARIUS'''<br />
 
-
==Overview==
+
==ADENYLATE KINASE FROM SULFOLOBUS ACIDOCALDARIUS==
-
The adenylate kinase from the hyperthermophilic archaean species Sulfolobus acidocaldarius has been cloned, expressed in Escherichia coli, purified and crystallized. The crystal structure was elucidated by multiple isomorphous replacement and non-crystallographic density averaging. The structure was refined at 2.6 A (1 A=0.1 nm) resolution. The enzyme is trimeric, in contrast to previous solution measurements that suggested a dimeric structure, and in contrast to the vast majority of adenylate kinases, which are monomeric. In large parts of each subunit the chain fold resembles the known enzyme structure from eubacteria and eukaryotes although the sequence homology is negligible. Since the asymmetric unit contains two trimers with and without bound AMP at the AMP sites and with an ADP at one of the six ATP sites, the analysis shows the enzyme in several states. The conformational differences between these states resemble those of other adenylate kinases. Because of sequence homology, the structure presented provides a good model for the methanococcal adenylate kinases.
+
<StructureSection load='1nks' size='340' side='right'caption='[[1nks]], [[Resolution|resolution]] 2.57&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1nks]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NKS FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.57&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nks FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nks OCA], [https://pdbe.org/1nks PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nks RCSB], [https://www.ebi.ac.uk/pdbsum/1nks PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nks ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KADA_SULAC KADA_SULAC]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nk/1nks_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nks ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1NKS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius] with <scene name='pdbligand=AMP:'>AMP</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenylate_kinase Adenylate kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.3 2.7.4.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NKS OCA].
+
*[[Adenylate kinase 3D structures|Adenylate kinase 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The structure of a trimeric archaeal adenylate kinase., Vonrhein C, Bonisch H, Schafer G, Schulz GE, J Mol Biol. 1998 Sep 11;282(1):167-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9733648 9733648]
+
[[Category: Large Structures]]
-
[[Category: Adenylate kinase]]
+
-
[[Category: Single protein]]
+
[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
-
[[Category: Schulz, G E.]]
+
[[Category: Schulz GE]]
-
[[Category: Vonrhein, C.]]
+
[[Category: Vonrhein C]]
-
[[Category: ADP]]
+
-
[[Category: AMP]]
+
-
[[Category: kinase]]
+
-
[[Category: thermophilic]]
+
-
[[Category: transferase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:07:12 2008''
+

Current revision

ADENYLATE KINASE FROM SULFOLOBUS ACIDOCALDARIUS

PDB ID 1nks

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nks"
Personal tools