3i78

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[[Image:3i78.png|left|200px]]
 
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{{STRUCTURE_3i78| PDB=3i78 | SCENE= }}
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==35/99/170/186/220-loops of FXa in SGT==
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<StructureSection load='3i78' size='340' side='right'caption='[[3i78]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3i78]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I78 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I78 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i78 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i78 OCA], [https://pdbe.org/3i78 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i78 RCSB], [https://www.ebi.ac.uk/pdbsum/3i78 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i78 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRYP_STRGR TRYP_STRGR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i7/3i78_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i78 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Converting one enzyme into another is challenging due to the uneven distribution of important amino acids for function in both protein sequence and structure. We report a strategy for protein engineering allowing an organized mixing and matching of genetic material that leverages lower throughput with increased quality of screens. Our approach successfully tested the contribution of each surface-exposed loop in the trypsin fold alone and the cooperativity of their combinations towards building the substrate selectivity and Na(+)-dependent allosteric activation of the protease domain of human coagulation factor Xa into a bacterial trypsin. As the created proteases lack additional protein domains and protein co-factor activation mechanism requisite for the complexity of blood coagulation, they are stepping-stones towards further understanding and engineering of artificial clotting factors.
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===35/99/170/186/220-loops of FXa in SGT===
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Combinatorial enzyme design probes allostery and cooperativity in the trypsin fold.,Page MJ, Di Cera E J Mol Biol. 2010 Jun 4;399(2):306-19. Epub 2010 Apr 24. PMID:20399789<ref>PMID:20399789</ref>
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{{ABSTRACT_PUBMED_20399789}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3i78" style="background-color:#fffaf0;"></div>
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[[3i78]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I78 OCA].
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==See Also==
==See Also==
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*[[Trypsin|Trypsin]]
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020399789</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces griseus]]
[[Category: Streptomyces griseus]]
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[[Category: Trypsin]]
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[[Category: Di Cera E]]
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[[Category: Cera, E Di.]]
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[[Category: Page MJ]]
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[[Category: Page, M J.]]
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[[Category: Beta sheet]]
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[[Category: Disulfide bond]]
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[[Category: Hydrolase]]
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[[Category: Protease]]
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[[Category: Serine protease]]
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[[Category: Zymogen]]
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Current revision

35/99/170/186/220-loops of FXa in SGT

PDB ID 3i78

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