1nnl

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[[Image:1nnl.gif|left|200px]]<br /><applet load="1nnl" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nnl, resolution 1.53&Aring;" />
 
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'''Crystal structure of Human Phosphoserine Phosphatase'''<br />
 
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==Overview==
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==Crystal structure of Human Phosphoserine Phosphatase==
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The crystal structure of human phosphoserine phosphatase (HPSP) in the open conformation has been determined at a resolution of 1.53 A. The crystals are orthorhombic, belonging to space group C222(1), with unit-cell parameters a = 49.03, b = 130.25, c = 157.29 A. The asymmetric unit contains two molecules. Phase information was derived from a multiwavelength anomalous dispersion (MAD) experiment conducted at three wavelengths using a selenomethionine-derivative crystal of HPSP. The structure was refined using CNS to a final crystallographic R value of 21.6% (R(free) = 23.4%). HPSP is a dimeric enzyme responsible for the third and final step of the l-serine biosynthesis pathway. It catalyses the Mg2+-dependent hydrolysis of l-phosphoserine. Recently, the structure of HPSP in complex with an inhibitor bound to the active site has been reported to be the open conformation of the enzyme. Here, the structure of HPSP is reported in the absence of substrate in the active site. Evidence is presented that HPSP in an uncomplexed form is in an even more open conformation than in the inhibitor complex. In this state, the enzyme is partially unfolded to allow the substrate to enter the active site. Binding of the substrate causes HPSP to shift to the closed conformation by stabilizing the partially unfolded region. In the present structure a Ca2+ ion is bound to the active site and an explanation is given why HPSP is not active when in the active site Mg2+ is replaced by a Ca2+ ion.
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<StructureSection load='1nnl' size='340' side='right'caption='[[1nnl]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nnl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NNL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nnl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nnl OCA], [https://pdbe.org/1nnl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nnl RCSB], [https://www.ebi.ac.uk/pdbsum/1nnl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nnl ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/SERB_HUMAN SERB_HUMAN] Defects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:[https://omim.org/entry/614023 614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome.<ref>PMID:14673469</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/SERB_HUMAN SERB_HUMAN] Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates.<ref>PMID:12777757</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nn/1nnl_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nnl ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NNL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Phosphoserine_phosphatase Phosphoserine phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.3 3.1.3.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NNL OCA].
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*[[Phosphoserine phosphatase|Phosphoserine phosphatase]]
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== References ==
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==Reference==
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<references/>
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High-resolution structure of human phosphoserine phosphatase in open conformation., Peeraer Y, Rabijns A, Verboven C, Collet JF, Van Schaftingen E, De Ranter C, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):971-7. Epub 2003, May 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12777757 12777757]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoserine phosphatase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Collet JF]]
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[[Category: Collet, J F.]]
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[[Category: De Ranter C]]
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[[Category: Peeraer, Y.]]
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[[Category: Peeraer Y]]
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[[Category: Rabijns, A.]]
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[[Category: Rabijns A]]
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[[Category: Ranter, C De.]]
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[[Category: Van Schaftingen E]]
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[[Category: Schaftingen, E Van.]]
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[[Category: Verboven C]]
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[[Category: Verboven, C.]]
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[[Category: CA]]
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[[Category: CL]]
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[[Category: hpsp]]
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[[Category: phospho-aspartyl]]
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[[Category: phosphoserine phosphatase]]
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[[Category: psp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:07:59 2008''
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Current revision

Crystal structure of Human Phosphoserine Phosphatase

PDB ID 1nnl

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