1nog

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[[Image:1nog.gif|left|200px]]<br /><applet load="1nog" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nog, resolution 1.55&Aring;" />
 
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'''Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum'''<br />
 
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==Overview==
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==Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum==
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ATP:cobalamin adenosyltransferase MMAB was recently identified as the gene responsible for a disorder of cobalamin metabolism in humans (cblB complementation group). The crystal structure of the MMAB sequence homologue from Thermoplasma acidophilum (TA1434; GenBank identification number gi|16082403) was determined to a resolution of 1.5 A. TA1434 was confirmed to be an ATP:cobalamin adenosyltransferase, which depended absolutely on divalent metal ions (Mg2+ &gt; Mn2+ &gt; Co2+) and only used ATP or dATP as adenosyl donors. The apparent Km of TA1434 was 110 microM (kcat = 0.23 s(-1)) for ATP, 140 microM (kcat = 0.11 s(-1)) for dATP, and 3 microM (kcat = 0.18 s(-1)) for cobalamin. TA1434 is a trimer in solution and in the crystal structure, with each subunit composed of a five-helix bundle. The location of disease-related point mutations and other residues conserved among the homologues of TA1434 suggest that the active site lies at the junctions between the subunits. Mutations in TA1434 that correspond to the disease-related mutations resulted in proteins that were inactive for ATP:cobalamin adenosyltransferase activity in vitro, confirming that these mutations define the molecular basis of the human disease.
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<StructureSection load='1nog' size='340' side='right'caption='[[1nog]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1nog]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NOG FirstGlance]. <br>
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1NOG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermoplasma_acidophilum Thermoplasma acidophilum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NOG OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nog FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nog OCA], [https://pdbe.org/1nog PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nog RCSB], [https://www.ebi.ac.uk/pdbsum/1nog PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nog ProSAT], [https://www.topsan.org/Proteins/MCSG/1nog TOPSAN]</span></td></tr>
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==Reference==
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</table>
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The structural basis for methylmalonic aciduria. The crystal structure of archaeal ATP:cobalamin adenosyltransferase., Saridakis V, Yakunin A, Xu X, Anandakumar P, Pennycooke M, Gu J, Cheung F, Lew JM, Sanishvili R, Joachimiak A, Arrowsmith CH, Christendat D, Edwards AM, J Biol Chem. 2004 May 28;279(22):23646-53. Epub 2004 Mar 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15044458 15044458]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/Q9HIA7_THEAC Q9HIA7_THEAC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/no/1nog_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nog ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermoplasma acidophilum]]
[[Category: Thermoplasma acidophilum]]
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[[Category: Arrowsmith, C H.]]
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[[Category: Arrowsmith CH]]
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[[Category: Christendat, D.]]
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[[Category: Christendat D]]
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[[Category: Edwards, A M.]]
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[[Category: Edwards AM]]
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[[Category: Gu, J.]]
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[[Category: Gu J]]
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[[Category: Iakounine, A.]]
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[[Category: Iakounine A]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Pennycooke M]]
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[[Category: Pennycooke, M.]]
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[[Category: Sanishvili R]]
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[[Category: Sanishvili, R.]]
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[[Category: Saridakis V]]
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[[Category: Saridakis, V.]]
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[[Category: Xu X]]
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[[Category: Xu, X.]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomics]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomics]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:14 2008''
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Current revision

Crystal Structure of Conserved Protein 0546 from Thermoplasma Acidophilum

PDB ID 1nog

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