This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1npo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:52, 10 April 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1npo.gif|left|200px]]<br /><applet load="1npo" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1npo, resolution 3.0&Aring;" />
 
-
'''BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN'''<br />
 
-
==Overview==
+
==BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN==
-
The first crystal structure of the pituitary hormone oxytocin complexed with its carrier protein neurophysin has been determined and refined to 3.0 A resolution. The hormone-binding site is located at the end of a 3(10)-helix and involves residues from both domains of each monomer. Hormone residues Tyr 2, which is buried deep in the binding pocket, and Cys 1 have been confirmed as the key residues involved in neurophysin-hormone recognition. We have compared the bound oxytocin observed in the neurophysin-oxytocin complex, the X-ray structures of unbound oxytocin analogues and the NMR-derived structure for bound oxytocin. We find that while our structure is in agreement with the previous crystallographic findings, it differs from the NMR result with regard to how Tyr 2 of the hormone is recognized by neurophysin.
+
<StructureSection load='1npo' size='340' side='right'caption='[[1npo]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1npo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NPO FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1npo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1npo OCA], [https://pdbe.org/1npo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1npo RCSB], [https://www.ebi.ac.uk/pdbsum/1npo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1npo ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/NEU2_BOVIN NEU2_BOVIN] Neurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/1npo_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1npo ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1NPO is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NPO OCA].
+
*[[Neurophysin|Neurophysin]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Crystal structure of the neurophysin-oxytocin complex., Rose JP, Wu CK, Hsiao CD, Breslow E, Wang BC, Nat Struct Biol. 1996 Feb;3(2):163-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8564543 8564543]
+
[[Category: Bos taurus]]
[[Category: Bos taurus]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
-
[[Category: Rose, J P.]]
+
[[Category: Rose JP]]
-
[[Category: Wang, B C.]]
+
[[Category: Wang B-C]]
-
[[Category: complex (hormone transport/hormone)]]
+
-
[[Category: hypothalamus]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:08:43 2008''
+

Current revision

BOVINE NEUROPHYSIN II COMPLEX WITH OXYTOCIN

PDB ID 1npo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1npo"
Personal tools