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1ntm

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[[Image:1ntm.gif|left|200px]]<br /><applet load="1ntm" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ntm, resolution 2.40&Aring;" />
 
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'''Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom'''<br />
 
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==Overview==
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==Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom==
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Cytochrome bc(1) is an integral membrane protein complex essential to cellular respiration and photosynthesis. The Q cycle reaction mechanism of bc(1) postulates a separated quinone reduction (Q(i)) and quinol oxidation (Q(o)) site. In a complete catalytic cycle, a quinone molecule at the Q(i) site receives two electrons from the b(H) heme and two protons from the negative side of the membrane; this process is specifically inhibited by antimycin A and NQNO. The structures of bovine mitochondrial bc(1) in the presence or absence of bound substrate ubiquinone and with either the bound antimycin A(1) or NQNO were determined and refined. A ubiquinone with its first two isoprenoid repeats and an antimycin A(1) were identified in the Q(i) pocket of the substrate and inhibitor bound structures, respectively; the NQNO, on the other hand, was identified in both Q(i) and Q(o) pockets in the inhibitor complex. The two inhibitors occupied different portions of the Q(i) pocket and competed with substrate for binding. In the Q(o) pocket, the NQNO behaves similarly to stigmatellin, inducing an iron-sulfur protein conformational arrest. Extensive binding interactions and conformational adjustments of residues lining the Q(i) pocket provide a structural basis for the high affinity binding of antimycin A and for phenotypes of inhibitor resistance. A two-water-mediated ubiquinone protonation mechanism is proposed involving three Q(i) site residues His(201), Lys(227), and Asp(228).
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<StructureSection load='1ntm' size='340' side='right'caption='[[1ntm]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ntm]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntm OCA], [https://pdbe.org/1ntm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntm RCSB], [https://www.ebi.ac.uk/pdbsum/1ntm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/QCR7_BOVIN QCR7_BOVIN] This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This component is involved in redox-linked proton pumping.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1ntm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ntm ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1NTM is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ubiquinol--cytochrome-c_reductase Ubiquinol--cytochrome-c reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.10.2.2 1.10.2.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTM OCA].
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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*[[Cytochrome bc1 3D structures|Cytochrome bc1 3D structures]]
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==Reference==
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__TOC__
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Structural basis for the quinone reduction in the bc1 complex: a comparative analysis of crystal structures of mitochondrial cytochrome bc1 with bound substrate and inhibitors at the Qi site., Gao X, Wen X, Esser L, Quinn B, Yu L, Yu CA, Xia D, Biochemistry. 2003 Aug 5;42(30):9067-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12885240 12885240]
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Ubiquinol--cytochrome-c reductase]]
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[[Category: Esser L]]
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[[Category: Esser, L.]]
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[[Category: Gao X]]
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[[Category: Gao, X.]]
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[[Category: Quinn B]]
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[[Category: Quinn, B.]]
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[[Category: Wen X]]
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[[Category: Wen, X.]]
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[[Category: Xia D]]
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[[Category: Xia, D.]]
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[[Category: Yu C]]
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[[Category: Yu, C.]]
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[[Category: Yu L]]
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[[Category: Yu, L.]]
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[[Category: FES]]
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[[Category: HEM]]
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[[Category: bc1]]
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[[Category: cytochrome b]]
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[[Category: cytochrome c1]]
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[[Category: electron transfer]]
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[[Category: iron sulfur protein]]
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[[Category: membrane protein]]
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[[Category: mitochondrial processing peptidase]]
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[[Category: mpp]]
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[[Category: oxidoreductase]]
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[[Category: protease]]
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[[Category: proton translocation]]
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[[Category: qcr]]
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[[Category: rieske]]
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[[Category: structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:52 2008''
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Current revision

Crystal Structure of Mitochondrial Cytochrome bc1 Complex at 2.4 Angstrom

PDB ID 1ntm

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