1ntv

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[[Image:1ntv.gif|left|200px]]<br /><applet load="1ntv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ntv, resolution 1.50&Aring;" />
 
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'''Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex'''<br />
 
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==Overview==
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==Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex==
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Formation of the mammalian six-layered neocortex depends on a signaling pathway that involves Reelin, the very low-density lipoprotein receptor, the apolipoprotein E receptor-2 (ApoER2), and the adaptor protein Disabled-1 (Dab1). The 1.5 A crystal structure of a complex between the Dab1 phosphotyrosine binding (PTB) domain and a 14-residue peptide from the ApoER2 tail explains the unusual preference of Dab1 for unphosphorylated tyrosine within the NPxY motif of the peptide. Crystals of the complex soaked with the phosphoinositide PI-4,5P(2) (PI) show that PI binds to conserved basic residues on the PTB domain opposite the peptide binding groove. This finding explains how the Dab1 PTB domain can simultaneously bind PI and the ApoER2 tail. Recruitment of the Dab1 PTB domain to PI-rich regions of the plasma membrane may facilitate association with the Reelin receptor cytoplasmic tails to transduce a critical positional cue to migrating neurons.
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<StructureSection load='1ntv' size='340' side='right'caption='[[1ntv]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ntv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NTV FirstGlance]. <br>
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1NTV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NTV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ntv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ntv OCA], [https://pdbe.org/1ntv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ntv RCSB], [https://www.ebi.ac.uk/pdbsum/1ntv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ntv ProSAT]</span></td></tr>
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Origins of peptide selectivity and phosphoinositide binding revealed by structures of disabled-1 PTB domain complexes., Stolt PC, Jeon H, Song HK, Herz J, Eck MJ, Blacklow SC, Structure. 2003 May;11(5):569-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12737822 12737822]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DAB1_MOUSE DAB1_MOUSE] Adapter molecule functioning in neural development. May regulate SIAH1 activity.<ref>PMID:9009273</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nt/1ntv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ntv ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Single protein]]
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[[Category: Blacklow SC]]
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[[Category: Blacklow, S C.]]
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[[Category: Eck MJ]]
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[[Category: Eck, M J.]]
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[[Category: Herz J]]
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[[Category: Herz, J.]]
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[[Category: Jeon H]]
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[[Category: Jeon, H.]]
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[[Category: Song HK]]
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[[Category: Song, H K.]]
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[[Category: Stolt PC]]
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[[Category: Stolt, P C.]]
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[[Category: PO4]]
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[[Category: beta-sandwich]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:09:56 2008''
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Current revision

Crystal Structure of the Disabled-1 (Dab1) PTB domain-ApoER2 peptide complex

PDB ID 1ntv

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