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1jq3

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[[Image:1jq3.png|left|200px]]
 
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{{STRUCTURE_1jq3| PDB=1jq3 | SCENE= }}
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==Crystal Structure of Spermidine Synthase in Complex with Transition State Analogue AdoDATO==
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<StructureSection load='1jq3' size='340' side='right'caption='[[1jq3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jq3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JQ3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AAT:S-ADENOSYL-1,8-DIAMINO-3-THIOOCTANE'>AAT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jq3 OCA], [https://pdbe.org/1jq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jq3 RCSB], [https://www.ebi.ac.uk/pdbsum/1jq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jq3 ProSAT], [https://www.topsan.org/Proteins/MCSG/1jq3 TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SPEE_THEMA SPEE_THEMA] Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine. It has lower affinity and lower activity towards 1,3-diaminopropane, cadaverine (1,5-diaminopentane), agmatine, thermine and spermidine (in vitro).<ref>PMID:17585781</ref> <ref>PMID:11731804</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jq/1jq3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jq3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Polyamines are essential in all branches of life. Spermidine synthase (putrescine aminopropyltransferase, PAPT) catalyzes the biosynthesis of spermidine, a ubiquitous polyamine. The crystal structure of the PAPT from Thermotoga maritima (TmPAPT) has been solved to 1.5 A resolution in the presence and absence of AdoDATO (S-adenosyl-1,8-diamino-3-thiooctane), a compound containing both substrate and product moieties. This, the first structure of an aminopropyltransferase, reveals deep cavities for binding substrate and cofactor, and a loop that envelops the active site. The AdoDATO binding site is lined with residues conserved in PAPT enzymes from bacteria to humans, suggesting a universal catalytic mechanism. Other conserved residues act sterically to provide a structural basis for polyamine specificity. The enzyme is tetrameric; each monomer consists of a C-terminal domain with a Rossmann-like fold and an N-terminal beta-stranded domain. The tetramer is assembled using a novel barrel-type oligomerization motif.
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===Crystal Structure of Spermidine Synthase in Complex with Transition State Analogue AdoDATO===
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The crystal structure of spermidine synthase with a multisubstrate adduct inhibitor.,Korolev S, Ikeguchi Y, Skarina T, Beasley S, Arrowsmith C, Edwards A, Joachimiak A, Pegg AE, Savchenko A Nat Struct Biol. 2002 Jan;9(1):27-31. PMID:11731804<ref>PMID:11731804</ref>
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{{ABSTRACT_PUBMED_11731804}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1jq3" style="background-color:#fffaf0;"></div>
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[[1jq3]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JQ3 OCA].
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==See Also==
==See Also==
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*[[Spermidine Synthase|Spermidine Synthase]]
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*[[Spermidine synthase 3D structures|Spermidine synthase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:011731804</ref><ref group="xtra">PMID:015340214</ref><references group="xtra"/>
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__TOC__
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[[Category: Spermidine synthase]]
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
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[[Category: Beasley, S.]]
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[[Category: Beasley S]]
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[[Category: Edwards, A.]]
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[[Category: Edwards A]]
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[[Category: Ikeguchi, Y.]]
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[[Category: Ikeguchi Y]]
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[[Category: Joachimiak, A.]]
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[[Category: Joachimiak A]]
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[[Category: Korolev, S.]]
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[[Category: Korolev S]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Pegg AE]]
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[[Category: Pegg, A E.]]
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[[Category: Savchenko A]]
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[[Category: Savchenko, A.]]
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[[Category: Skarina T]]
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[[Category: Skarina, T.]]
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[[Category: Aminopropyltransferase]]
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[[Category: Beta-barrel]]
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[[Category: Homo-tetramer]]
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[[Category: Mcsg]]
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[[Category: Midwest center for structural genomic]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Thermophyle]]
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[[Category: Transferase]]
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[[Category: Transition-state analogue]]
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Current revision

Crystal Structure of Spermidine Synthase in Complex with Transition State Analogue AdoDATO

PDB ID 1jq3

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