2keq

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[[Image:2keq.png|left|200px]]
 
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{{STRUCTURE_2keq| PDB=2keq | SCENE= }}
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==Solution structure of DnaE intein from Nostoc punctiforme==
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<StructureSection load='2keq' size='340' side='right'caption='[[2keq]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2keq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_punctiforme_PCC_73102 Nostoc punctiforme PCC 73102]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KEQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2keq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2keq OCA], [https://pdbe.org/2keq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2keq RCSB], [https://www.ebi.ac.uk/pdbsum/2keq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2keq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2J066_NOSP7 B2J066_NOSP7] [https://www.uniprot.org/uniprot/B2J821_NOSP7 B2J821_NOSP7]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ke/2keq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2keq ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated.
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===Solution structure of DnaE intein from Nostoc punctiforme===
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Solution structure of DnaE intein from Nostoc punctiforme: structural basis for the design of a new split intein suitable for site-specific chemical modification.,Oeemig JS, Aranko AS, Djupsjobacka J, Heinamaki K, Iwai H FEBS Lett. 2009 May 6;583(9):1451-6. Epub 2009 Apr 1. PMID:19344715<ref>PMID:19344715</ref>
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{{ABSTRACT_PUBMED_19344715}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2keq" style="background-color:#fffaf0;"></div>
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[[2keq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_punctiforme_pcc_73102 Nostoc punctiforme pcc 73102]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KEQ OCA].
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==See Also==
==See Also==
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*[[DNA polymerase|DNA polymerase]]
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*[[DNA polymerase 3D structures|DNA polymerase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019344715</ref><references group="xtra"/>
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__TOC__
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[[Category: Nostoc punctiforme pcc 73102]]
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</StructureSection>
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[[Category: Aranko, A S.]]
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[[Category: Large Structures]]
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[[Category: Djupsj, J B.]]
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[[Category: Nostoc punctiforme PCC 73102]]
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[[Category: Iwai, H.]]
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[[Category: Aranko AS]]
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[[Category: Oeemig, J S.]]
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[[Category: Djupsj JB]]
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[[Category: Dnae intein]]
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[[Category: Iwai H]]
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[[Category: Intein]]
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[[Category: Oeemig JS]]
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[[Category: Protein trans splicing]]
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[[Category: Splicing]]
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Current revision

Solution structure of DnaE intein from Nostoc punctiforme

PDB ID 2keq

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