1nwk

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[[Image:1nwk.gif|left|200px]]<br /><applet load="1nwk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1nwk, resolution 1.85&Aring;" />
 
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'''CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE==
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A nucleotide-dependent conformational change regulates actin filament dynamics. Yet, the structural basis of this mechanism remains controversial. The x-ray crystal structure of tetramethylrhodamine-5-maleimide-actin with bound AMPPNP, a non-hydrolyzable ATP analog, was determined to 1.85-A resolution. A comparison of this structure to that of tetramethylrhodamine-5-maleimide-actin with bound ADP, determined previously under similar conditions, reveals how the release of the nucleotide gamma-phosphate sets in motion a sequence of events leading to a conformational change in subdomain 2. The side chain of Ser-14 in the catalytic site rotates upon Pi release, triggering the rearrangement of the loop containing the methylated His-73, referred to as the sensor loop. This in turn causes a transition in the DNase I-binding loop in subdomain 2 from a disordered loop in ATP-actin to an ordered alpha-helix in ADP-actin. Despite this conformational change, the nucleotide cleft remains closed in ADP-actin, similar to ATP-actin. An analysis of the existing structures of members of the actin superfamily suggests that the cleft is open in the nucleotide-free state.
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<StructureSection load='1nwk' size='340' side='right'caption='[[1nwk]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1nwk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NWK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=RHO:TETRAMETHYLRHODAMINE-5-MALEIMIDE'>RHO</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nwk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nwk OCA], [https://pdbe.org/1nwk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nwk RCSB], [https://www.ebi.ac.uk/pdbsum/1nwk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nwk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ACTS_RABIT ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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==About this Structure==
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==See Also==
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1NWK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus] with <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=ANP:'>ANP</scene> and <scene name='pdbligand=RHO:'>RHO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NWK OCA].
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*[[Actin 3D structures|Actin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of monomeric actin in the ATP state. Structural basis of nucleotide-dependent actin dynamics., Graceffa P, Dominguez R, J Biol Chem. 2003 Sep 5;278(36):34172-80. Epub 2003 Jun 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12813032 12813032]
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[[Category: Large Structures]]
[[Category: Oryctolagus cuniculus]]
[[Category: Oryctolagus cuniculus]]
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[[Category: Single protein]]
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[[Category: Dominguez R]]
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[[Category: Dominguez, R.]]
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[[Category: Graceffa P]]
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[[Category: Graceffa, P.]]
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[[Category: ANP]]
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[[Category: CA]]
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[[Category: RHO]]
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[[Category: actin]]
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[[Category: amppnp]]
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[[Category: atp-state]]
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[[Category: tetramethylrhodamine-5-maleimide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:10:51 2008''
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Current revision

CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE

PDB ID 1nwk

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