2zzx

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[[Image:2zzx.png|left|200px]]
 
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{{STRUCTURE_2zzx| PDB=2zzx | SCENE= }}
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==Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate==
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<StructureSection load='2zzx' size='340' side='right'caption='[[2zzx]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2zzx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZZX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAC:LACTIC+ACID'>LAC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zzx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zzx OCA], [https://pdbe.org/2zzx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zzx RCSB], [https://www.ebi.ac.uk/pdbsum/2zzx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zzx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TLBP_THET8 TLBP_THET8] Part of the tripartite ATP-independent periplasmic (TRAP) transport system involved in the uptake of lactate. This protein specifically binds L-lactate.[UniProtKB:P37676]<ref>PMID:19631222</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zz/2zzx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zzx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lactate is utilized in many biological processes, and its transport across biological membranes is mediated with various types of transporters. Here, we report the crystal structures of a lactate-binding protein of a TRAP (tripartite ATP-independent periplasmic) secondary transporter from Thermus thermophilus HB8. The folding of the protein is typical for a type II periplasmic solute-binding protein and forms a dimer in a back-to-back manner. One molecule of l-lactate is clearly identified in a cleft of the protein as a complex with a calcium ion. Detailed crystallographic and biochemical analyses revealed that the calcium ion can be removed from the protein and replaced with other divalent cations. This characterization of the structure of a protein binding with calcium lactate makes a significant contribution to our understanding of the mechanisms by which calcium and lactate are accommodated in cells.
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===Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate===
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Crystal structure of a periplasmic substrate-binding protein in complex with calcium lactate.,Akiyama N, Takeda K, Miki K J Mol Biol. 2009 Sep 25;392(3):559-65. Epub 2009 Jul 22. PMID:19631222<ref>PMID:19631222</ref>
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{{ABSTRACT_PUBMED_19631222}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2zzx" style="background-color:#fffaf0;"></div>
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[[2zzx]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZZX OCA].
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==See Also==
==See Also==
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*[[ABC transporter|ABC transporter]]
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*[[ABC transporter 3D structures|ABC transporter 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019631222</ref><references group="xtra"/>
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__TOC__
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[[Category: Thermus thermophilus]]
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</StructureSection>
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[[Category: Akiyama, N.]]
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[[Category: Large Structures]]
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[[Category: Miki, K.]]
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[[Category: Thermus thermophilus HB8]]
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[[Category: Takeda, K.]]
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[[Category: Akiyama N]]
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[[Category: Lactate]]
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[[Category: Miki K]]
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[[Category: Periplasmic substrate binding protein]]
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[[Category: Takeda K]]
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[[Category: Transport protein]]
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[[Category: Trap transporter]]
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Current revision

Crystal Structure of a Periplasmic Substrate Binding Protein in Complex with Lactate

PDB ID 2zzx

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