4f3t

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[[Image:4f3t.png|left|200px]]
 
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{{STRUCTURE_4f3t| PDB=4f3t | SCENE= }}
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==Human Argonaute-2 - miR-20a complex==
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<StructureSection load='4f3t' size='340' side='right'caption='[[4f3t]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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===Human Argonaute-2 - miR-20a complex===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4f3t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F3T FirstGlance]. <br>
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{{ABSTRACT_PUBMED_22682761}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPH:PHENOL'>IPH</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f3t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f3t OCA], [https://pdbe.org/4f3t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f3t RCSB], [https://www.ebi.ac.uk/pdbsum/4f3t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f3t ProSAT]</span></td></tr>
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[[4f3t]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F3T OCA].
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AGO2_HUMAN AGO2_HUMAN] Required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also up-regulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and up-regulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.<ref>PMID:15105377</ref> <ref>PMID:15260970</ref> <ref>PMID:15337849</ref> <ref>PMID:15284456</ref> <ref>PMID:16271387</ref> <ref>PMID:16289642</ref> <ref>PMID:16142218</ref> <ref>PMID:16357216</ref> <ref>PMID:15800637</ref> <ref>PMID:16081698</ref> <ref>PMID:16936728</ref> <ref>PMID:16756390</ref> <ref>PMID:17382880</ref> <ref>PMID:17524464</ref> <ref>PMID:17932509</ref> <ref>PMID:17531811</ref> <ref>PMID:17507929</ref> <ref>PMID:18048652</ref> <ref>PMID:18771919</ref> <ref>PMID:18690212</ref> <ref>PMID:18178619</ref> <ref>PMID:19167051</ref>
==See Also==
==See Also==
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*[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
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*[[Argonaute 3D structures|Argonaute 3D structures]]
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*[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:022682761</ref><references group="xtra"/>
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Elkayam, E.]]
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[[Category: Large Structures]]
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[[Category: Joshua-Tor, L.]]
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[[Category: Elkayam E]]
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[[Category: Kuhn, C D.]]
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[[Category: Joshua-Tor L]]
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[[Category: Tocilj, A.]]
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[[Category: Kuhn C-D]]
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[[Category: Hydrolase-rna complex]]
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[[Category: Tocilj A]]
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[[Category: Hydrolase/gene regulation]]
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[[Category: Rna]]
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[[Category: Rnai]]
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[[Category: Slicer]]
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Current revision

Human Argonaute-2 - miR-20a complex

PDB ID 4f3t

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