2bhx

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[[Image:2bhx.png|left|200px]]
 
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{{STRUCTURE_2bhx| PDB=2bhx | SCENE= }}
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==Radiation damage of the Schiff base in phosphoserine aminotransferase (structure A)==
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<StructureSection load='2bhx' size='340' side='right'caption='[[2bhx]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2bhx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_alcalophilus Alkalihalobacillus alcalophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BHX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bhx OCA], [https://pdbe.org/2bhx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bhx RCSB], [https://www.ebi.ac.uk/pdbsum/2bhx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bhx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SERC_ALKAL SERC_ALKAL] Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine.<ref>PMID:14646107</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bh/2bhx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bhx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The X-ray susceptibility of the lysine-pyridoxal-5'-phosphate Schiff base in Bacillus alcalophilus phosphoserine aminotransferase has been investigated using crystallographic data collected at 100 K to 1.3 A resolution, complemented by on-line spectroscopic studies. X-rays induce deprotonation of the internal aldimine, changes in the Schiff base conformation, displacement of the cofactor molecule, and disruption of the Schiff base linkage between pyridoxal-5'-phosphate and the Lys residue. Analysis of the "undamaged" structure reveals a significant chemical strain on the internal aldimine bond that leads to a pronounced geometrical distortion of the cofactor. However, upon crystal exposure to the X-rays, the strain and distortion are relaxed and eventually diminished when the total absorbed dose has exceeded 4.7 x 10(6) Ggamma. Our data provide new insights into the enzymatic activation of pyridoxal-5'-phosphate and suggest that special care should be taken while using macromolecular crystallography to study details in strained active sites.
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===RADIATION DAMAGE OF THE SCHIFF BASE IN PHOSPHOSERINE AMINOTRANSFERASE (STRUCTURE A)===
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Strain relief at the active site of phosphoserine aminotransferase induced by radiation damage.,Dubnovitsky AP, Ravelli RB, Popov AN, Papageorgiou AC Protein Sci. 2005 Jun;14(6):1498-507. Epub 2005 May 9. PMID:15883191<ref>PMID:15883191</ref>
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{{ABSTRACT_PUBMED_15883191}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2bhx" style="background-color:#fffaf0;"></div>
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[[2bhx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_alcalophilus Bacillus alcalophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BHX OCA].
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==See Also==
==See Also==
*[[Phosphoserine aminotransferase|Phosphoserine aminotransferase]]
*[[Phosphoserine aminotransferase|Phosphoserine aminotransferase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015883191</ref><references group="xtra"/>
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__TOC__
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[[Category: Bacillus alcalophilus]]
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</StructureSection>
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[[Category: Phosphoserine transaminase]]
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[[Category: Alkalihalobacillus alcalophilus]]
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[[Category: Dubnovitsky, A P.]]
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[[Category: Large Structures]]
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[[Category: Papageorgiou, A C.]]
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[[Category: Dubnovitsky AP]]
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[[Category: Popov, A N.]]
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[[Category: Papageorgiou AC]]
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[[Category: Ravelli, R B.G.]]
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[[Category: Popov AN]]
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[[Category: Aminotransferase]]
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[[Category: Ravelli RBG]]
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[[Category: Pyridoxal-5'-phosphate]]
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[[Category: Radiation damage]]
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[[Category: Transferase]]
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Current revision

Radiation damage of the Schiff base in phosphoserine aminotransferase (structure A)

PDB ID 2bhx

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