2e5m

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[[Image:2e5m.png|left|200px]]
 
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{{STRUCTURE_2e5m| PDB=2e5m | SCENE= }}
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==Crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain 7==
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<StructureSection load='2e5m' size='340' side='right'caption='[[2e5m]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2e5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfurisphaera_tokodaii_str._7 Sulfurisphaera tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2E5M FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2e5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2e5m OCA], [https://pdbe.org/2e5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2e5m RCSB], [https://www.ebi.ac.uk/pdbsum/2e5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2e5m ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q96YK6_SULTO Q96YK6_SULTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/e5/2e5m_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2e5m ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Isocitrate dehydrogenase is a catabolic enzyme that acts during the third step of the tricarboxylic acid cycle. The hypothetical protein ST2166 from the archaeon Sulfolobus tokodaii was isolated and crystallized. It shares high primary structure homology with prokaryotic NADP(+)-dependent IDHs, suggesting that these enzymes share a common enzymatic mechanism. The crystal structure of ST2166 was determined at 2.0 A resolution in the apo form, and then the structure of the crystal soaked with NADP(+) was also determined at 2.4 A resolution, which contained NADP(+) bound at the putative active site. Comparisons between the structures of apo and NADP(+)-bound forms and NADP-IDHs from other prokaryotes suggest that prokaryotic NADP-IDHs recognize their cofactors using conserved Lys335, Tyr336, and Arg386 in ST2166 at the opening cleft before the domain closure.
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===Crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain 7===
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Crystal Structures of the Putative Isocitrate Dehydrogenase from Sulfolobus tokodaii Strain 7 in the Apo and NADP(+)-Bound Forms.,Kondo H, Murakami M Archaea. 2018 Dec 19;2018:7571984. doi: 10.1155/2018/7571984. eCollection 2018. PMID:30662370<ref>PMID:30662370</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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[[2e5m]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_tokodaii_str._7 Sulfolobus tokodaii str. 7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2E5M OCA].
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<div class="pdbe-citations 2e5m" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
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*[[Ferguson sandbox 1|Ferguson sandbox 1]]
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*[[Isocitrate dehydrogenase 3D structures|Isocitrate dehydrogenase 3D structures]]
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*[[Isocitrate dehydrogenase|Isocitrate dehydrogenase]]
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== References ==
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[[Category: Sulfolobus tokodaii str. 7]]
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<references/>
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[[Category: Kondo, H.]]
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__TOC__
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[[Category: Kouyama, T.]]
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</StructureSection>
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[[Category: Nadp-dependent isocitrate dehydrogenase]]
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[[Category: Large Structures]]
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[[Category: Oxidoreductase]]
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[[Category: Sulfurisphaera tokodaii str. 7]]
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[[Category: Kondo H]]
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[[Category: Kouyama T]]

Current revision

Crystal structure of isocitrate dehydrogenase from Sulfolobus tokodaii strain 7

PDB ID 2e5m

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