1xji

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[[Image:1xji.png|left|200px]]
 
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{{STRUCTURE_1xji| PDB=1xji | SCENE= }}
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==Bacteriorhodopsin crystallized in bicelles at room temperature==
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<StructureSection load='1xji' size='340' side='right'caption='[[1xji]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1xji]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XJI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C14:TETRADECANE'>C14</scene>, <scene name='pdbligand=CPS:3-[(3-CHOLAMIDOPROPYL)DIMETHYLAMMONIO]-1-PROPANESULFONATE'>CPS</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xji OCA], [https://pdbe.org/1xji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xji RCSB], [https://www.ebi.ac.uk/pdbsum/1xji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xji ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xj/1xji_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xji ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We showed previously that high-quality crystals of bacteriorhodopsin (bR) from Halobacterium salinarum can be obtained from bicelle-forming DMPC/CHAPSO mixtures at 37 degrees C. As many membrane proteins are not sufficiently stable for crystallization at this high temperature, we tested whether the bicelle method could be applied at a lower temperature. Here we show that bR can be crystallized at room temperature using two different bicelle-forming compositions: DMPC/CHAPSO and DTPC/CHAPSO. The DTPC/CHAPSO crystals grown at room temperature are essentially identical to the previous, twinned crystals: space group P21 with unit cell dimensions of a = 44.7 A, b = 108.7 A, c = 55.8 A, beta = 113.6 degrees . The room-temperature DMPC/CHAPSO crystals are untwinned, however, and belong to space group C222(1) with the following unit cell dimensions: a = 44.7 A, b = 102.5 A, c = 128.2 A. The bR protein packs into almost identical layers in the two crystal forms, but the layers stack differently. The new untwinned crystal form yielded clear density for a previously unresolved CHAPSO molecule inserted between protein subunits within the layers. The ability to grow crystals at room temperature significantly expands the applicability of bicelle crystallization.
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===Bacteriorhodopsin crystallized in bicelles at room temperature===
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Crystallization of bacteriorhodopsin from bicelle formulations at room temperature.,Faham S, Boulting GL, Massey EA, Yohannan S, Yang D, Bowie JU Protein Sci. 2005 Mar;14(3):836-40. Epub 2005 Feb 2. PMID:15689517<ref>PMID:15689517</ref>
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{{ABSTRACT_PUBMED_15689517}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1xji" style="background-color:#fffaf0;"></div>
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[[1xji]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJI OCA].
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==See Also==
==See Also==
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*[[Bacteriorhodopsin|Bacteriorhodopsin]]
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*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:015689517</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Halobacterium salinarum]]
[[Category: Halobacterium salinarum]]
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[[Category: Boulting, G L.]]
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[[Category: Large Structures]]
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[[Category: Bowie, J U.]]
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[[Category: Boulting GL]]
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[[Category: Faham, S.]]
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[[Category: Bowie JU]]
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[[Category: Massey, E A.]]
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[[Category: Faham S]]
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[[Category: Yang, D.]]
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[[Category: Massey EA]]
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[[Category: Yohannan, S.]]
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[[Category: Yang D]]
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[[Category: Membrane protein]]
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[[Category: Yohannan S]]
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[[Category: Membrane protein bacteriorhodopsin]]
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Current revision

Bacteriorhodopsin crystallized in bicelles at room temperature

PDB ID 1xji

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