2k4t

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[[Image:2k4t.png|left|200px]]
 
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{{STRUCTURE_2k4t| PDB=2k4t | SCENE= }}
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==Solution structure of human VDAC-1 in LDAO micelles==
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<StructureSection load='2k4t' size='340' side='right'caption='[[2k4t]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2k4t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K4T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k4t OCA], [https://pdbe.org/2k4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k4t RCSB], [https://www.ebi.ac.uk/pdbsum/2k4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k4t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VDAC1_HUMAN VDAC1_HUMAN] Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.<ref>PMID:11845315</ref> <ref>PMID:15033708</ref> <ref>PMID:18755977</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k4/2k4t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k4t ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The voltage-dependent anion channel (VDAC) mediates trafficking of small molecules and ions across the eukaryotic outer mitochondrial membrane. VDAC also interacts with antiapoptotic proteins from the Bcl-2 family, and this interaction inhibits release of apoptogenic proteins from the mitochondrion. We present the nuclear magnetic resonance (NMR) solution structure of recombinant human VDAC-1 reconstituted in detergent micelles. It forms a 19-stranded beta barrel with the first and last strand parallel. The hydrophobic outside perimeter of the barrel is covered by detergent molecules in a beltlike fashion. In the presence of cholesterol, recombinant VDAC-1 can form voltage-gated channels in phospholipid bilayers similar to those of the native protein. NMR measurements revealed the binding sites of VDAC-1 for the Bcl-2 protein Bcl-x(L), for reduced beta-nicotinamide adenine dinucleotide, and for cholesterol. Bcl-x(L) interacts with the VDAC barrel laterally at strands 17 and 18.
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===Solution structure of human VDAC-1 in LDAO micelles===
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Solution structure of the integral human membrane protein VDAC-1 in detergent micelles.,Hiller S, Garces RG, Malia TJ, Orekhov VY, Colombini M, Wagner G Science. 2008 Aug 29;321(5893):1206-10. PMID:18755977<ref>PMID:18755977</ref>
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{{ABSTRACT_PUBMED_18755977}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 2k4t" style="background-color:#fffaf0;"></div>
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[[2k4t]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K4T OCA].
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==See Also==
==See Also==
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*[[Porin|Porin]]
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018755977</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Colombini, M.]]
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[[Category: Large Structures]]
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[[Category: Garces, R G.]]
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[[Category: Colombini M]]
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[[Category: Hiller, S.]]
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[[Category: Garces RG]]
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[[Category: Malia, T J.]]
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[[Category: Hiller S]]
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[[Category: Orekhov, V Y.]]
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[[Category: Malia TJ]]
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[[Category: Wagner, G.]]
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[[Category: Orekhov VY]]
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[[Category: Apoptosis]]
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[[Category: Wagner G]]
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[[Category: Host-virus interaction]]
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[[Category: Ion transport]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Mitochondrion]]
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[[Category: Outer membrane]]
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[[Category: Phosphoprotein]]
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[[Category: Porin]]
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[[Category: Transmembrane]]
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[[Category: Transport protein]]
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Current revision

Solution structure of human VDAC-1 in LDAO micelles

PDB ID 2k4t

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