3i61

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[[Image:3i61.png|left|200px]]
 
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{{STRUCTURE_3i61| PDB=3i61 | SCENE= }}
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==Structure of Mss116p bound to ssRNA and ADP-Beryllium Fluoride==
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<StructureSection load='3i61' size='340' side='right'caption='[[3i61]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3i61]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I61 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I61 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i61 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i61 OCA], [https://pdbe.org/3i61 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i61 RCSB], [https://www.ebi.ac.uk/pdbsum/3i61 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i61 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MS116_YEAST MS116_YEAST] ATP-dependent RNA helicase required for mitochondrial splicing of group I and II introns. Specifically involved in the ATP-dependent splicing of the bl1 intron of COB. Also required for efficient mitochondrial translation.<ref>PMID:2535893</ref> <ref>PMID:7567443</ref> <ref>PMID:12402239</ref> <ref>PMID:15618406</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/3i61_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i61 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The yeast DEAD box protein Mss116p is a general RNA chaperone that functions in mitochondrial group I and II intron splicing, translational activation, and RNA end processing. Here we determined high-resolution X-ray crystal structures of Mss116p complexed with an RNA oligonucleotide and ATP analogs AMP-PNP, ADP-BeF(3)(-), or ADP-AlF(4)(-). The structures show the entire helicase core acting together with a functionally important C-terminal extension. In all structures, the helicase core is in a closed conformation with a wedge alpha helix bending RNA 3' of the central bound nucleotides, as in previous DEAD box protein structures. Notably, Mss116p's C-terminal extension also bends RNA 5' of the central nucleotides, resulting in RNA crimping. Despite reported functional differences, we observe few structural changes in ternary complexes with different ATP analogs. The structures constrain models of DEAD box protein function and reveal a strand separation mechanism in which a protein uses two wedges to act as a molecular crimper.
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===Structure of Mss116p bound to ssRNA and ADP-Beryllium Fluoride===
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Structure of the Yeast DEAD box protein Mss116p reveals two wedges that crimp RNA.,Del Campo M, Lambowitz AM Mol Cell. 2009 Sep 11;35(5):598-609. PMID:19748356<ref>PMID:19748356</ref>
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{{ABSTRACT_PUBMED_19748356}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 3i61" style="background-color:#fffaf0;"></div>
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[[3i61]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I61 OCA].
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==See Also==
==See Also==
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*[[Helicase|Helicase]]
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019748356</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Campo, M Del.]]
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[[Category: Del Campo M]]
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[[Category: Lambowitz, A M.]]
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[[Category: Lambowitz AM]]
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[[Category: Atp-binding]]
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[[Category: Dead-box]]
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[[Category: Helicase]]
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[[Category: Hydrolase]]
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[[Category: Hydrolase-rna complex]]
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[[Category: Mitochondrion]]
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[[Category: Mrna processing]]
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[[Category: Mrna splicing]]
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[[Category: Nucleotide-binding]]
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[[Category: Protein-rna complex]]
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[[Category: Rna helicase]]
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[[Category: Rna-binding]]
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[[Category: Transit peptide]]
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[[Category: Translation regulation]]
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Current revision

Structure of Mss116p bound to ssRNA and ADP-Beryllium Fluoride

PDB ID 3i61

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