1oa1

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[[Image:1oa1.jpg|left|200px]]<br /><applet load="1oa1" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oa1, resolution 1.55&Aring;" />
 
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'''REDUCED HYBRID CLUSTER PROTEIN (HCP) FROM DESULFOVIBRIO VULGARIS HILDENBOROUGH STRUCTURE AT 1.55A RESOLUTION USING SYNCHROTRON RADIATION.'''<br />
 
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==Overview==
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==REDUCED HYBRID CLUSTER PROTEIN (HCP) FROM DESULFOVIBRIO VULGARIS HILDENBOROUGH STRUCTURE AT 1.55A RESOLUTION USING SYNCHROTRON RADIATION.==
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The hybrid cluster proteins from the sulfate reducing bacteria Desulfovibrio desulfuricans ATCC 27774 ( Dd) and Desulfovibrio vulgaris strain Hildenborough ( Dv) have been isolated and crystallized anaerobically. In each case, the protein has been reduced with dithionite and the crystal structure of the reduced form elucidated using X-ray synchrotron radiation techniques at 1.25 A and 1.55 A resolution for Dd and Dv, respectively. Although the overall structures of the proteins are unchanged upon reduction, there are significant changes at the hybrid cluster centres. These include significant movements in the position of the iron atom linked to the persulfide moiety in the oxidized as-isolated proteins and the sulfur atom of the persulfide itself. The nature of these changes is described and the implications with respect to the function of hybrid cluster proteins are discussed.
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<StructureSection load='1oa1' size='340' side='right'caption='[[1oa1]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oa1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OA1 FirstGlance]. <br>
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1OA1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris] with <scene name='pdbligand=SF4:'>SF4</scene>, <scene name='pdbligand=SF3:'>SF3</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OA1 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF3:FE4-S3+CLUSTER'>SF3</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oa1 OCA], [https://pdbe.org/1oa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oa1 RCSB], [https://www.ebi.ac.uk/pdbsum/1oa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oa1 ProSAT]</span></td></tr>
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Reduced hybrid cluster proteins (HCP) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at high resolution using synchrotron radiation., Aragao D, Macedo S, Mitchell EP, Romao CV, Liu MY, Frazao C, Saraiva LM, Xavier AV, LeGall J, van Dongen WM, Hagen WR, Teixeira M, Carrondo MA, Lindley P, J Biol Inorg Chem. 2003 May;8(5):540-8. Epub 2003 Feb 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12764602 12764602]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HCP_DESVH HCP_DESVH] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oa/1oa1_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oa1 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Desulfovibrio vulgaris]]
[[Category: Desulfovibrio vulgaris]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Aragao, D.]]
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[[Category: Aragao D]]
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[[Category: Carrondo, M A.]]
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[[Category: Carrondo MA]]
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[[Category: Dongen, W M.A M.Van.]]
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[[Category: Frazao C]]
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[[Category: Frazao, C.]]
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[[Category: Hagen WR]]
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[[Category: Hagen, W R.]]
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[[Category: Legall J]]
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[[Category: Legall, J.]]
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[[Category: Lindley PF]]
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[[Category: Lindley, P F.]]
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[[Category: Liu MY]]
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[[Category: Liu, M Y.]]
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[[Category: Macedo S]]
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[[Category: Macedo, S.]]
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[[Category: Mitchell EP]]
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[[Category: Mitchell, E P.]]
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[[Category: Romao CV]]
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[[Category: Romao, C V.]]
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[[Category: Saraiva LM]]
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[[Category: Saraiva, L M.]]
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[[Category: Teixeira M]]
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[[Category: Teixeira, M.]]
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[[Category: Van Dongen WMAM]]
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[[Category: Xavier, A V.]]
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[[Category: Xavier AV]]
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[[Category: GOL]]
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[[Category: SF3]]
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[[Category: SF4]]
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[[Category: desulfovibrio vulgaris]]
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[[Category: hybrid cluster protein]]
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[[Category: reduced forms]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:15:12 2008''
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Current revision

REDUCED HYBRID CLUSTER PROTEIN (HCP) FROM DESULFOVIBRIO VULGARIS HILDENBOROUGH STRUCTURE AT 1.55A RESOLUTION USING SYNCHROTRON RADIATION.

PDB ID 1oa1

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