We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

2ads

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "2ads" [edit=sysop:move=sysop])
Current revision (14:39, 17 November 2021) (edit) (undo)
 
(6 intermediate revisions not shown.)
Line 1: Line 1:
{{Theoretical_model}}
{{Theoretical_model}}
-
[[Image:2ads.png|left|200px]]
+
==THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.==
 +
<StructureSection load='2ads' size='340' side='right'caption='[[2ads]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ADS FirstGlance]. <br>
 +
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ads FirstGlance], [https://www.ebi.ac.uk/pdbsum/2ads PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ads ProSAT]</span></td></tr>
 +
</table>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
-
{{STRUCTURE_2ads| PDB=2ads | SCENE= }}
+
Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage.,Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186<ref>PMID:16061186</ref>
-
===THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.===
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
{{ABSTRACT_PUBMED_16061186}}
+
<div class="pdbe-citations 2ads" style="background-color:#fffaf0;"></div>
-
 
+
== References ==
-
==Reference==
+
<references/>
-
<ref group="xtra">PMID:016061186</ref><references group="xtra"/>
+
__TOC__
 +
</StructureSection>
 +
[[Category: Theoretical Model]]
 +
[[Category: Large Structures]]
[[Category: Cheng, H Y]]
[[Category: Cheng, H Y]]
[[Category: Dauter, Z]]
[[Category: Dauter, Z]]

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

THEORETICAL MODEL OF DNA-MEDIATED RNA CLEAVAGE BY A.AEOLICUS ARGONAUTE PROTEIN.

PDB ID 2ads

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ads"
Personal tools