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4sod

From Proteopedia

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{{Theoretical_model}}
{{Theoretical_model}}
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[[Image:4sod.png|left|200px]]
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==RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE==
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<StructureSection load='4sod' size='340' side='right'caption='[[4sod]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4SOD FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4sod FirstGlance], [https://www.ebi.ac.uk/pdbsum/4sod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4sod ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In order to improve the therapeutic effectiveness of human Cu,Zn superoxide dismutase (HSOD) by targeting it to cell surfaces and increasing its circulatory half-life, we have designed and expressed a heparin-binding derivative of HSOD. This design was based on the idea that structurally independent protein units, HSOD and the heparin-binding A+ helix from protein C inhibitor, could be combined with a carefully chosen linker, GlyProGly, to form a stable, bifunctional protein. The chimeric HSOD-GlyProGly-A+ protein was expressed and secreted to the periplasm of E. coli and had normal SOD activity. HSOD-GlyProGly-A+ had a significantly increased retention time relative to wild-type HSOD on a heparin affinity column, indicating that it was successfully targeted to heparin, and this binding was maintained at physiological ionic strength. When administered to mice, HSOD-GlyProGly-A+ had a half-life of approximately 15 minutes, twice that of wild-type HSOD. Our rational design approach should be generally applicable to the creation of bifunctional chimeric molecules.
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{{STRUCTURE_4sod| PDB=4sod | SCENE= }}
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Rational design and expression of a heparin-targeted human superoxide dismutase.,Boissinot M, Kuhn LA, Lee P, Fisher CL, Wang Y, Hallewell RA, Tainer JA Biochem Biophys Res Commun. 1993 Jan 15;190(1):250-6. PMID:8422249<ref>PMID:8422249</ref>
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===RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE===
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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{{ABSTRACT_PUBMED_8422249}}
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<div class="pdbe-citations 4sod" style="background-color:#fffaf0;"></div>
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:008422249</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
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[[Category: Theoretical Model]]
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[[Category: Large Structures]]
[[Category: Fisher, C L]]
[[Category: Fisher, C L]]
[[Category: Kuhn, L A]]
[[Category: Kuhn, L A]]
[[Category: Tainer, J A]]
[[Category: Tainer, J A]]

Current revision

Theoretical Model: The protein structure described on this page was determined theoretically, and hence should be interpreted with caution.

RATIONAL DESIGN AND EXPRESSION OF A HEPARIN-TARGETED HUMAN SUPEROXIDE DISMUTASE

PDB ID 4sod

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