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1of9

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[[Image:1of9.jpg|left|200px]]<br /><applet load="1of9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1of9" />
 
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'''SOLUTION STRUCTURE OF THE PORE FORMING TOXIN OF ENTAMOEBA HISTOLYTICA (AMOEBAPORE A)'''<br />
 
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==Overview==
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==Solution structure of the pore forming toxin of entamoeba histolytica (Amoebapore A)==
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Amoebapore A is a 77-residue protein from the protozoan parasite and human pathogen Entamoeba histolytica. Amoebapores lyse both bacteria and eukaryotic cells by pore formation and play a pivotal role in the destruction of host tissues during amoebiasis, one of the most life-threatening parasitic diseases. Amoebapore A belongs to the superfamily of saposin-like proteins that are characterized by a conserved disulfide bond pattern and a fold consisting of five helices. Membrane-permeabilizing effector molecules of mammalian lymphocytes such as porcine NK-lysin and the human granulysin share these structural attributes. Several mechanisms have been proposed to explain how saposin-like proteins form membrane pores. All mechanisms indicate that the surface charge distribution of these proteins is the basis of their membrane binding capacity and pore formation. Here, we have solved the structure of amoebapore A by NMR spectroscopy. We demonstrate that the specific activation step of amoebapore A depends on a pH-dependent dimerization event and is modulated by a surface-exposed histidine residue. Thus, histidine-mediated dimerization is the molecular switch for pore formation and reveals a novel activation mechanism of pore-forming toxins.
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<StructureSection load='1of9' size='340' side='right'caption='[[1of9]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1of9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF9 FirstGlance]. <br>
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1OF9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Entamoeba_histolytica Entamoeba histolytica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of9 OCA], [https://pdbe.org/1of9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of9 RCSB], [https://www.ebi.ac.uk/pdbsum/1of9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of9 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of the pore-forming protein of Entamoeba histolytica., Hecht O, Van Nuland NA, Schleinkofer K, Dingley AJ, Bruhn H, Leippe M, Grotzinger J, J Biol Chem. 2004 Apr 23;279(17):17834-41. Epub 2004 Feb 17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14970207 14970207]
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== Function ==
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[https://www.uniprot.org/uniprot/PFPA_ENTHI PFPA_ENTHI] Forms pores in the cytoplasmic membrane of host cells. Has antibacterial activity against M.luteus, no activity against E.coli. Implicated in the cytolytic activity of the parasite.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1of9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1of9 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Entamoeba histolytica]]
[[Category: Entamoeba histolytica]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bruhn, H.]]
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[[Category: Bruhn H]]
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[[Category: Dingley, A J.]]
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[[Category: Dingley AJ]]
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[[Category: Grotzinger, J.]]
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[[Category: Grotzinger J]]
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[[Category: Hecht, O.]]
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[[Category: Hecht O]]
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[[Category: Leippe, M.]]
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[[Category: Leippe M]]
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[[Category: Nuland, N Van.]]
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[[Category: Schleinkofer K]]
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[[Category: Schleinkofer, K.]]
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[[Category: Van Nuland N]]
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[[Category: amoebapore a]]
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[[Category: pore forming]]
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[[Category: saplip]]
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[[Category: toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:17:03 2008''
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Current revision

Solution structure of the pore forming toxin of entamoeba histolytica (Amoebapore A)

PDB ID 1of9

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