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1aof

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[[Image:1aof.gif|left|200px]]<br />
 
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<applet load="1aof" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aof, resolution 2.0&Aring;" />
 
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'''CYTOCHROME CD1 NITRITE REDUCTASE, REDUCED FORM'''<br />
 
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==Overview==
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==CYTOCHROME CD1 NITRITE REDUCTASE, REDUCED FORM==
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Cytochrome cd1 nitrite reductase catalyses the conversion of nitrite to, nitric oxide in the nitrogen cycle. The crystal structure of the oxidized, enzyme shows that the d1 haem iron of the active site is ligated by, His/Tyr side chains, and the c haem iron is ligated by a His/His ligand, pair. Here we show that both haems undergo re-ligation during catalysis., Upon reduction, the tyrosine ligand of the d1 haem is released to allow, substrate binding. Concomitantly, a refolding of the cytochrome c domain, takes place, resulting in an unexpected change of the c haem iron, coordination from His 17/His 69 to Met106/His69. This step is similar to, the last steps in the folding of cytochrome c. The changes must affect the, redox potential of the haems, and suggest a mechanism by which ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9311786 (full description)]]
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<StructureSection load='1aof' size='340' side='right'caption='[[1aof]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aof]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_pantotrophus Paracoccus pantotrophus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AOF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AOF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DHE:HEME+D'>DHE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO2:SULFUR+DIOXIDE'>SO2</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aof FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aof OCA], [https://pdbe.org/1aof PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aof RCSB], [https://www.ebi.ac.uk/pdbsum/1aof PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aof ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NIRS_PARPN NIRS_PARPN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ao/1aof_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aof ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1AOF is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Paracoccus_pantotrophus Paracoccus pantotrophus]] with HEM, DHE and SO2 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Sites: C1A, C1B, D1A and D1B. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AOF OCA]].
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*[[Cytochrome c nitrite reductase|Cytochrome c nitrite reductase]]
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__TOC__
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==Reference==
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</StructureSection>
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Haem-ligand switching during catalysis in crystals of a nitrogen-cycle enzyme., Williams PA, Fulop V, Garman EF, Saunders NF, Ferguson SJ, Hajdu J, Nature. 1997 Sep 25;389(6649):406-12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9311786 9311786]
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[[Category: Large Structures]]
[[Category: Paracoccus pantotrophus]]
[[Category: Paracoccus pantotrophus]]
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[[Category: Single protein]]
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[[Category: Fulop V]]
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[[Category: Fulop, V.]]
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[[Category: Williams PA]]
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[[Category: Williams, P.A.]]
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[[Category: DHE]]
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[[Category: HEM]]
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[[Category: SO2]]
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[[Category: enzyme]]
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[[Category: nitrate assimilation]]
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[[Category: nitrite reductase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:50:18 2007''
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Current revision

CYTOCHROME CD1 NITRITE REDUCTASE, REDUCED FORM

PDB ID 1aof

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