1oko

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[[Image:1oko.gif|left|200px]]<br /><applet load="1oko" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oko, resolution 1.600&Aring;" />
 
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'''CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA LECTIN 1 COMPLEXED WITH GALACTOSE AT 1.6 A RESOLUTION'''<br />
 
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==Overview==
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==Crystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolution==
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The structure of the tetrameric Pseudomonas aeruginosa lectin I (PA-IL) in complex with galactose and calcium was determined at 1.6 A resolution, and the native protein was solved at 2.4 A resolution. Each monomer adopts a beta-sandwich fold with ligand binding site at the apex. All galactose hydroxyl groups, except O1, are involved in a hydrogen bond network with the protein and O3 and O4 also participate in the co-ordination of the calcium ion. The stereochemistry of calcium galactose binding is reminiscent of that observed in some animal C-type lectins. The structure of the complex provides a framework for future design of anti-bacterial compounds.
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<StructureSection load='1oko' size='340' side='right'caption='[[1oko]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1oko]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OKO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oko OCA], [https://pdbe.org/1oko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oko RCSB], [https://www.ebi.ac.uk/pdbsum/1oko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oko ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
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==About this Structure==
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==See Also==
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1OKO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with <scene name='pdbligand=GAL:'>GAL</scene>, <scene name='pdbligand=CA:'>CA</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=MPD:'>MPD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Glb+Binding+Site+For+Chain+D'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OKO OCA].
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*[[Galactose-binding lectin|Galactose-binding lectin]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural basis of calcium and galactose recognition by the lectin PA-IL of Pseudomonas aeruginosa., Cioci G, Mitchell EP, Gautier C, Wimmerova M, Sudakevitz D, Perez S, Gilboa-Garber N, Imberty A, FEBS Lett. 2003 Dec 4;555(2):297-301. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14644431 14644431]
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Cioci G]]
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[[Category: Cioci, G.]]
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[[Category: Gautier C]]
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[[Category: Gautier, C.]]
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[[Category: Gilboa-Garber N]]
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[[Category: Gilboa-Garber, N.]]
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[[Category: Imberty A]]
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[[Category: Imberty, A.]]
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[[Category: Mitchell E]]
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[[Category: Mitchell, E.]]
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[[Category: Perez S]]
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[[Category: Perez, S.]]
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[[Category: Wimmerova M]]
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[[Category: Wimmerova, M.]]
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[[Category: CA]]
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[[Category: GAL]]
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[[Category: MPD]]
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[[Category: SO4]]
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[[Category: galactose binding]]
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[[Category: lectin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:18:51 2008''
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Current revision

Crystal structure of Pseudomonas Aeruginosa Lectin 1 complexed with galactose at 1.6 A resolution

PDB ID 1oko

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