1oo0

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[[Image:1oo0.gif|left|200px]]<br /><applet load="1oo0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1oo0, resolution 1.85&Aring;" />
 
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'''Crystal structure of the Drosophila Mago nashi-Y14 complex'''<br />
 
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==Overview==
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==Crystal structure of the Drosophila Mago nashi-Y14 complex==
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Pre-mRNA splicing is essential for generating mature mRNA and is also important for subsequent mRNA export and quality control. The splicing history is imprinted on spliced mRNA through the deposition of a splicing-dependent multiprotein complex, the exon junction complex (EJC), at approximately 20 nucleotides upstream of exon-exon junctions. The EJC is a dynamic structure containing proteins functioning in the nuclear export and nonsense-mediated decay of spliced mRNAs. Mago nashi (Mago) and Y14 are core components of the EJC, and they form a stable heterodimer that strongly associates with spliced mRNA. Here we report a 1.85 A-resolution structure of the Drosophila Mago-Y14 complex. Surprisingly, the structure shows that the canonical RNA-binding surface of the Y14 RNA recognition motif (RRM) is involved in extensive protein-protein interactions with Mago. This unexpected finding provides important insights for understanding the molecular mechanisms of EJC assembly and RRM-mediated protein-protein interactions.
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<StructureSection load='1oo0' size='340' side='right'caption='[[1oo0]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1oo0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OO0 FirstGlance]. <br>
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1OO0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=SR:'>SR</scene>, <scene name='pdbligand=BME:'>BME</scene>, <scene name='pdbligand=MPD:'>MPD</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OO0 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1oo0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1oo0 OCA], [https://pdbe.org/1oo0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1oo0 RCSB], [https://www.ebi.ac.uk/pdbsum/1oo0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1oo0 ProSAT]</span></td></tr>
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Crystal structure of the Drosophila Mago nashi-Y14 complex., Shi H, Xu RM, Genes Dev. 2003 Apr 15;17(8):971-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12704080 12704080]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MGN_DROME MGN_DROME] Participates in the bidirectional intercellular signaling between the posterior follicle cells and oocyte to establish spatial coordinates that induces axis formation. Complex with tsu is essential for cytoplasmic localization of oskar in the posterior pole of oocytes. Required for the polarization of the oocyte microtubule cytoskeleton.<ref>PMID:8026338</ref> <ref>PMID:9272960</ref> <ref>PMID:9210377</ref> <ref>PMID:12730685</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oo/1oo0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1oo0 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Shi, H.]]
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[[Category: Shi H]]
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[[Category: Xu, R M.]]
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[[Category: Xu RM]]
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[[Category: BME]]
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[[Category: GOL]]
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[[Category: MPD]]
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[[Category: SR]]
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[[Category: exon junction complex]]
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[[Category: protein complex]]
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[[Category: rna recognition motif]]
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[[Category: splicing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:19:51 2008''
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Current revision

Crystal structure of the Drosophila Mago nashi-Y14 complex

PDB ID 1oo0

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