1ci5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Protected "1ci5" [edit=sysop:move=sysop])
Current revision (23:26, 27 December 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ci5.png|left|200px]]
 
-
{{STRUCTURE_1ci5| PDB=1ci5 | SCENE= }}
+
==GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)==
 +
<StructureSection load='1ci5' size='340' side='right'caption='[[1ci5]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ci5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CI5 FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ci5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ci5 OCA], [https://pdbe.org/1ci5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ci5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ci5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ci5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/LFA3_HUMAN LFA3_HUMAN] Ligand of the T-lymphocyte CD2 glycoprotein. This interaction is important in mediating thymocyte interactions with thymic epithelial cells, antigen-independent and -dependent interactions of T-lymphocytes with target cells and antigen-presenting cells and the T-lymphocyte rosetting with erythrocytes. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ci/1ci5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ci5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
A general strategy is presented here for producing glycan-free forms of glycoproteins without loss of function by employing apolar-to-polar mutations of surface residues in functionally irrelevant epitopes. The success of this structure-based approach was demonstrated through the expression in Escherichia coli of a soluble 11 kDa adhesion domain extracted from the heavily glycosylated 55 kDa human CD58 ectodomain. The solution structure was subsequently determined and binding to its counter-receptor CD2 studied by NMR. This mutant adhesion domain is functional as determined by several experimental methods, and the size of its binding site has been probed by chemical shift perturbations in NMR titration experiments. The new structural information supports a 'hand-shake' model of CD2-CD58 interaction involving the GFCC'C" faces of both CD2 and CD58 adhesion domains. The region responsible for binding specificity is most likely localized on the C, C' and C" strands and the C-C' and C'-C" loops on CD58.
-
===GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)===
+
Functional glycan-free adhesion domain of human cell surface receptor CD58: design, production and NMR studies.,Sun ZY, Dotsch V, Kim M, Li J, Reinherz EL, Wagner G EMBO J. 1999 Jun 1;18(11):2941-9. PMID:10357807<ref>PMID:10357807</ref>
-
{{ABSTRACT_PUBMED_10357807}}
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
 
+
</div>
-
==About this Structure==
+
<div class="pdbe-citations 1ci5" style="background-color:#fffaf0;"></div>
-
[[1ci5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CI5 OCA].
+
== References ==
-
 
+
<references/>
-
==Reference==
+
__TOC__
-
<ref group="xtra">PMID:010357807</ref><references group="xtra"/>
+
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Dotsch, V.]]
+
[[Category: Large Structures]]
-
[[Category: Kim, M.]]
+
[[Category: Dotsch V]]
-
[[Category: Li, J.]]
+
[[Category: Kim M]]
-
[[Category: Reinherz, E L.]]
+
[[Category: Li J]]
-
[[Category: Sun, Z Y.J.]]
+
[[Category: Reinherz EL]]
-
[[Category: Wagner, G.]]
+
[[Category: Sun ZYJ]]
-
[[Category: Adhesion glycoprotein]]
+
[[Category: Wagner G]]
-
[[Category: Cell surface receptor]]
+
-
[[Category: Immune system]]
+
-
[[Category: Immunoglobulin superfamily v-set domain]]
+

Current revision

GLYCAN-FREE MUTANT ADHESION DOMAIN OF HUMAN CD58 (LFA-3)

PDB ID 1ci5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ci5"
Personal tools