1c5e

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[[Image:1c5e.png|left|200px]]
 
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{{STRUCTURE_1c5e| PDB=1c5e | SCENE= }}
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==BACTERIOPHAGE LAMBDA HEAD PROTEIN D==
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<StructureSection load='1c5e' size='340' side='right'caption='[[1c5e]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1c5e]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C5E FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c5e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c5e OCA], [https://pdbe.org/1c5e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c5e RCSB], [https://www.ebi.ac.uk/pdbsum/1c5e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c5e ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DECO_LAMBD DECO_LAMBD] Stabilizes the expansion of the capsid head shell after genome packaging. The packaging of viral genome in the procapsid triggers a dramatic reconfiguration of the capsid shell, expanding from roughly 50nm to 60nm while the capsid thickness decreases. 415 capsid decoration protein molecules cooperatively bind the expanded capsid, thereby stabilizing the mature capsid shell.<ref>PMID:18786402</ref> <ref>PMID:21821043</ref> <ref>PMID:8411174</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c5/1c5e_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c5e ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of gpD, the capsid-stabilizing protein of bacteriophage lambda, was solved at 1.1 A resolution. Data were obtained from twinned crystals in space group P21 and refined with anisotropic temperature factors to an R-factor of 0.098 (Rfree = 0. 132). GpD (109 residues) has a novel fold with an unusually low content of regular secondary structure. Noncrystallographic trimers with substantial intersubunit interfaces were observed. The C-termini are well ordered and located on one side of the trimer, relatively far from its three-fold axis. The N-termini are disordered up to Ser 15, which is close to the three-fold axis and on the same side as the C-termini. A density map of the icosahedral viral capsid at 15 A resolution, obtained by cryo-electron microscopy and image reconstruction, reveals gpD trimers, seemingly indistinguishable from the ones seen in the crystals, at all three-fold sites. The map further reveals that the side of the trimer that binds to the capsid is the side on which both termini reside. Despite this orientation of the gpD trimer, fusion proteins connected by linker peptides to either terminus bind to the capsid, allowing protein and peptide display.
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===BACTERIOPHAGE LAMBDA HEAD PROTEIN D===
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Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD.,Yang F, Forrer P, Dauter Z, Conway JF, Cheng N, Cerritelli ME, Steven AC, Pluckthun A, Wlodawer A Nat Struct Biol. 2000 Mar;7(3):230-7. PMID:10700283<ref>PMID:10700283</ref>
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{{ABSTRACT_PUBMED_10700283}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==About this Structure==
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<div class="pdbe-citations 1c5e" style="background-color:#fffaf0;"></div>
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[[1c5e]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C5E OCA].
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== References ==
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<references/>
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==Reference==
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__TOC__
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<ref group="xtra">PMID:010700283</ref><references group="xtra"/>
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</StructureSection>
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[[Category: Enterobacteria phage lambda]]
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[[Category: Escherichia virus Lambda]]
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[[Category: Dauter, Z.]]
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[[Category: Large Structures]]
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[[Category: Forrer, P.]]
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[[Category: Dauter Z]]
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[[Category: Pluckthun, A.]]
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[[Category: Forrer P]]
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[[Category: Wlodawer, A.]]
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[[Category: Pluckthun A]]
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[[Category: Yang, F.]]
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[[Category: Wlodawer A]]
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[[Category: Bacteriophage lambda]]
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[[Category: Yang F]]
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[[Category: Head protein d]]
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[[Category: Phage display]]
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[[Category: Protein crystal structure]]
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[[Category: Viral protein]]
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[[Category: Virus assembly]]
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Current revision

BACTERIOPHAGE LAMBDA HEAD PROTEIN D

PDB ID 1c5e

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