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1ovn

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[[Image:1ovn.jpg|left|200px]]<br /><applet load="1ovn" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ovn, resolution 1.90&Aring;" />
 
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'''Crystal Structure and Functional Analysis of Drosophila Wind-- a PDI-Related Protein'''<br />
 
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==Overview==
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==Crystal Structure and Functional Analysis of Drosophila Wind-- a PDI-Related Protein==
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In the developing Drosophila melanogaster embryo, dorsal-ventral patterning displays an absolute requirement for the product of the essential windbeutel gene, Wind. In homozygous windbeutel mutant flies, dorsal-ventral patterning fails to initiate because of the failure of the Golgi-resident proteoglycan-modifying protein, Pipe, to exit the endoplasmic reticulum, and this leads to the death of the embryo. Here, we describe the three-dimensional structure of Wind at 1.9-A resolution and identify a candidate surface for interaction with Pipe. This represents the first crystal structure of a eukaryotic protein-disulfide isomerase-related protein of the endoplasmic reticulum to be described. The dimeric protein is composed of an N-terminal thioredoxin domain and a C-terminal alpha-helical domain unique to protein-disulfide isomerase D proteins. Although Wind carries a CXXC motif that is partially surface accessible, this motif is redox inactive, and the cysteines are not required for the targeting of Pipe to the Golgi. However, both domains are required for targeting Pipe to the Golgi, and, although the mouse homologue ERp28 cannot replace the function of Wind, exchange of the Wind D-domain with that of ERp28 allows for efficient Golgi transport of Pipe.
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<StructureSection load='1ovn' size='340' side='right'caption='[[1ovn]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ovn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OVN FirstGlance]. <br>
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1OVN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=CS:'>CS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OVN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS:CESIUM+ION'>CS</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ovn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ovn OCA], [https://pdbe.org/1ovn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ovn RCSB], [https://www.ebi.ac.uk/pdbsum/1ovn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ovn ProSAT]</span></td></tr>
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Crystal structure and functional analysis of Drosophila Wind, a protein-disulfide isomerase-related protein., Ma Q, Guo C, Barnewitz K, Sheldrick GM, Soling HD, Uson I, Ferrari DM, J Biol Chem. 2003 Nov 7;278(45):44600-7. Epub 2003 Aug 26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12941941 12941941]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/WBL_DROME WBL_DROME] Probable chaperone protein involved in dorsoventral axis patterning in early embryos. Probably acts by folding and targeting pipe (pip) into the Golgi.<ref>PMID:9568717</ref> <ref>PMID:11076773</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ov/1ovn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ovn ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barnewitz, K.]]
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[[Category: Barnewitz K]]
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[[Category: Ferrari, D M.]]
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[[Category: Ferrari DM]]
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[[Category: Guo, C.]]
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[[Category: Guo C]]
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[[Category: Ma, Q.]]
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[[Category: Ma Q]]
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[[Category: Sheldrick, G M.]]
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[[Category: Sheldrick GM]]
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[[Category: Soling, H D.]]
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[[Category: Soling HD]]
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[[Category: Uson, I.]]
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[[Category: Uson I]]
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[[Category: CS]]
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[[Category: chaperone]]
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[[Category: dorsal-ventral patterning]]
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[[Category: pdi]]
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[[Category: pdi-dbeta]]
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[[Category: pipe]]
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[[Category: protein disulfide isomerase]]
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[[Category: wind]]
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[[Category: windbeutel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:22:06 2008''
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Current revision

Crystal Structure and Functional Analysis of Drosophila Wind-- a PDI-Related Protein

PDB ID 1ovn

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