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1ozb

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[[Image:1ozb.gif|left|200px]]<br /><applet load="1ozb" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ozb, resolution 2.80&Aring;" />
 
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'''Crystal Structure of SecB complexed with SecA C-terminus'''<br />
 
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==Overview==
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==Crystal Structure of SecB complexed with SecA C-terminus==
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SecB is a bacterial chaperone involved in directing pre-protein to the translocation pathway by its specific interaction with the peripheral membrane ATPase SecA. The SecB-binding site on SecA is located at its C terminus and consists of a stretch of highly conserved residues. The crystal structure of SecB in complex with the C-terminal 27 amino acids of SecA from Haemophilus influenzae shows that the SecA peptide is structured as a CCCH zinc-binding motif. One SecB tetramer is bound by two SecA peptides, and the interface involves primarily salt bridges and hydrogen bonding interactions. The structure explains the importance of the zinc-binding motif and conserved residues at the C terminus of SecA in its high-affinity binding with SecB. It also suggests a model of SecB-SecA interaction and its implication for the mechanism of pre-protein transfer in bacterial protein translocation.
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<StructureSection load='1ozb' size='340' side='right'caption='[[1ozb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ozb]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozb OCA], [https://pdbe.org/1ozb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozb RCSB], [https://www.ebi.ac.uk/pdbsum/1ozb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SECB_HAEIN SECB_HAEIN] One of the proteins required for the normal export of preproteins out of the cell cytoplasm. It is a molecular chaperone that binds to a subset of precursor proteins, maintaining them in a translocation-competent state. It also specifically binds to its receptor SecA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/oz/1ozb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ozb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1OZB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZB OCA].
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*[[Preprotein translocase 3D structures|Preprotein translocase 3D structures]]
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*[[SecA|SecA]]
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==Reference==
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__TOC__
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Structural determinants of SecB recognition by SecA in bacterial protein translocation., Zhou J, Xu Z, Nat Struct Biol. 2003 Nov;10(11):942-7. Epub 2003 Sep 28. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14517549 14517549]
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</StructureSection>
[[Category: Haemophilus influenzae]]
[[Category: Haemophilus influenzae]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Xu, Z.]]
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[[Category: Xu Z]]
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[[Category: Zhou, J.]]
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[[Category: Zhou J]]
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[[Category: ZN]]
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[[Category: protein-protein complex]]
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[[Category: zinc binding motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:23:20 2008''
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Current revision

Crystal Structure of SecB complexed with SecA C-terminus

PDB ID 1ozb

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