4dat

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[[Image:4dat.png|left|200px]]
 
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{{STRUCTURE_4dat| PDB=4dat | SCENE= }}
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==Structure of 14-3-3 sigma in complex with PADI6 14-3-3 binding motif II==
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<StructureSection load='4dat' size='340' side='right'caption='[[4dat]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4dat]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DAT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dat OCA], [https://pdbe.org/4dat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dat RCSB], [https://www.ebi.ac.uk/pdbsum/4dat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dat ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The regulation and function of peptidylarginine deiminase isoform VI (PAD6), which is a highly abundant protein associated with the cytoplasmic lattices in mammalian oocytes, is poorly understood so far. It has been shown previously, that 14-3-3 proteins, a class of regulatory adapter proteins ubiquitous in eukaryotes, bind to PAD6 in vivo in a phosphorylation dependant manner. Here we identify possible 14-3-3 binding sites in human PAD6 by in silico methods, looking for conserved, surface exposed serine residues. Two of these sites were confirmed as 14-3-3 binding sites by fluorescence polarization competition and X-ray crystallography. We furthermore suggest a role of RSK-type kinases in the phosphorylation of one of these two binding sites and provide evidence in the form of in vitro kinase assays with p70S6 kinase and RSK1.
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===Structure of 14-3-3 sigma in complex with PADI6 14-3-3 binding motif II===
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Identification and structural characterization of two 14-3-3 binding sites in the human peptidylarginine deiminase type VI.,Rose R, Rose M, Ottmann C J Struct Biol. 2012 May 24. PMID:22634725<ref>PMID:22634725</ref>
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{{ABSTRACT_PUBMED_22634725}}
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4dat" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[4dat]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DAT OCA].
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*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:022634725</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein-arginine deiminase]]
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[[Category: Large Structures]]
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[[Category: Ottmann, C.]]
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[[Category: Ottmann C]]
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[[Category: Rose, M.]]
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[[Category: Rose M]]
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[[Category: Rose, R.]]
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[[Category: Rose R]]
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[[Category: 14-3-3 fold]]
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[[Category: Protein-protein interaction]]
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[[Category: Signaling protein-protein binding complex]]
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Current revision

Structure of 14-3-3 sigma in complex with PADI6 14-3-3 binding motif II

PDB ID 4dat

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