4h6d
From Proteopedia
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| - | [[Image:4h6d.png|left|200px]] | ||
| - | + | ==Crystal structure of PLP-soaked HMP synthase Thi5 from S. cerevisiae== | |
| + | <StructureSection load='4h6d' size='340' side='right'caption='[[4h6d]], [[Resolution|resolution]] 2.90Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4h6d]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4H6D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4H6D FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4h6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h6d OCA], [https://pdbe.org/4h6d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4h6d RCSB], [https://www.ebi.ac.uk/pdbsum/4h6d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4h6d ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/THI5_YEAST THI5_YEAST] Proposed biosynthetic enzyme involved in pyrimidine biosynthesis pathway above the hydroxymethyl-pyrimidine precursor leading to the thiamine moiety.<ref>PMID:12777485</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Vitamin B1 is essential for all organisms being well recognized as a necessary cofactor for key metabolic pathways such as glycolysis and was more recently implicated in DNA damage responses. Little is known about the enzyme responsible for the formation of the pyrimidine moiety (HMP-P synthase). We report a structure-function study of the HMP-P synthase from yeast, THI5p. Our crystallographic structure shows that the THI5p is a mix between periplasmic binding proteins and pyridoxal 5'-phosphate (PLP) dependent enzymes. Mutational and yeast complementation studies identify the key residues for HMP-P biosynthesis and offers unprecedented insights into the use of PLP as a substrate rather than as a cofactor, the requirement of iron and more generally into the HMP-P synthase reaction mechanism. | ||
| - | + | The last piece in the vitamin B1 biosynthesis puzzle: Structural and functional insight into yeast HMP-P synthase.,Coquille S, Roux C, Fitzpatrick TB, Thore S J Biol Chem. 2012 Oct 9. PMID:23048037<ref>PMID:23048037</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4h6d" style="background-color:#fffaf0;"></div> | ||
| - | == | + | ==See Also== |
| - | [[ | + | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Coquille | + | [[Category: Coquille SC]] |
| - | [[Category: Fitzpatrick | + | [[Category: Fitzpatrick T]] |
| - | [[Category: Roux | + | [[Category: Roux C]] |
| - | [[Category: Thore | + | [[Category: Thore S]] |
| - | + | ||
| - | + | ||
Current revision
Crystal structure of PLP-soaked HMP synthase Thi5 from S. cerevisiae
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