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1p6p

From Proteopedia

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[[Image:1p6p.jpg|left|200px]]<br /><applet load="1p6p" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p6p, resolution 2.5&Aring;" />
 
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'''Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein'''<br />
 
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==Overview==
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==Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein==
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Two paralogous groups of fatty acid-binding proteins (FABPs) have been described in vertebrate liver: liver FABP (L-FABP) type, extensively characterized in mammals, and liver basic FABP (Lb-FABP) found in fish, amphibians, reptiles, and birds. We describe here the toad Lb-FABP complete amino acid sequence, its X-ray structure to 2.5 A resolution, ligand-binding properties, and mechanism of fatty acid transfer to phospholipid membranes. Alignment of the amino acid sequence of toad Lb-FABP with known L-FABPs and Lb-FABPs shows that it is more closely related to the other Lb-FABPs. Toad Lb-FABP conserves the 12 characteristic residues present in all Lb-FABPs and absent in L-FABPs and presents the canonical fold characteristic of all the members of this protein family. Eight out of the 12 conserved residues point to the lipid-binding cavity of the molecule. In contrast, most of the 25 L-FABP conserved residues are in clusters on the surface of the molecule. The helix-turn-helix motif shows both a negative and positive electrostatic potential surface as in rat L-FABP, and in contrast with the other FABP types. The mechanism of anthroyloxy-labeled fatty acids transfer from Lb-FABP to phospholipid membranes occurs by a diffusion-mediated process, as previously shown for L-FABP, but the rate of transfer is 1 order of magnitude faster. Toad Lb-FABP can bind two cis-parinaric acid molecules but only one trans-parinaric acid molecule while L-FABP binds two molecules of both parinaric acid isomers. Although toad Lb-FABP shares with L-FABP a broad ligand-binding specificity, the relative affinity is different.
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<StructureSection load='1p6p' size='340' side='right'caption='[[1p6p]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p6p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhinella_arenarum Rhinella arenarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P6P FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p6p OCA], [https://pdbe.org/1p6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p6p RCSB], [https://www.ebi.ac.uk/pdbsum/1p6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p6p ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FABPL_RHIAE FABPL_RHIAE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p6/1p6p_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p6p ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1P6P is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bufo_arenarum Bufo arenarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P6P OCA].
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*[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structural and biochemical characterization of toad liver fatty acid-binding protein., Di Pietro SM, Corsico B, Perduca M, Monaco HL, Santome JA, Biochemistry. 2003 Jul 15;42(27):8192-203. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12846568 12846568]
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[[Category: Large Structures]]
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[[Category: Bufo arenarum]]
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[[Category: Rhinella arenarum]]
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[[Category: Single protein]]
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[[Category: Corsico B]]
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[[Category: Corsico, B.]]
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[[Category: Di Pietro SM]]
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[[Category: Monaco, H L.]]
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[[Category: Monaco HL]]
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[[Category: Perduca, M.]]
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[[Category: Perduca M]]
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[[Category: Pietro, S M.Di.]]
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[[Category: Santome JA]]
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[[Category: Santome, J A.]]
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[[Category: beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:47 2008''
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Current revision

Crystal Structure of Toad Liver Basic Fatty Acid-Binding Protein

PDB ID 1p6p

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