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1p7d

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[[Image:1p7d.gif|left|200px]]<br /><applet load="1p7d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p7d, resolution 2.95&Aring;" />
 
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'''Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA'''<br />
 
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==Overview==
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==Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA==
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The bacteriophage lambda integrase protein (lambda Int) belongs to a family of tyrosine recombinases that catalyze DNA rearrangements. We have determined a crystal structure of lambda Int complexed with a cleaved DNA substrate through a covalent phosphotyrosine bond. In comparison to an earlier unliganded structure, we observe a drastic conformational change in DNA-bound lambda Int that brings Tyr342 into the active site for cleavage of the DNA in cis. A flexible linker connects the central and the catalytic domains, allowing the protein to encircle the DNA. Binding specificity is achieved through direct interactions with the DNA and indirect readout of the flexibility of the att site. The conformational switch that activates lambda Int for DNA cleavage exposes the C-terminal 8 residues for interactions with a neighboring Int molecule. The protein interactions mediated by lambda Int's C-terminal tail offer a mechanism for the allosteric control of cleavage activity in higher order lambda Int complexes.
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<StructureSection load='1p7d' size='340' side='right'caption='[[1p7d]], [[Resolution|resolution]] 2.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p7d]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P7D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7d OCA], [https://pdbe.org/1p7d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p7d RCSB], [https://www.ebi.ac.uk/pdbsum/1p7d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p7d ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VINT_LAMBD VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p7/1p7d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p7d ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1P7D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P7D OCA].
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*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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A conformational switch controls the DNA cleavage activity of lambda integrase., Aihara H, Kwon HJ, Nunes-Duby SE, Landy A, Ellenberger T, Mol Cell. 2003 Jul;12(1):187-98. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12887904 12887904]
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[[Category: Escherichia virus Lambda]]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Aihara H]]
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[[Category: Aihara, H.]]
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[[Category: Ellenberger T]]
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[[Category: Ellenberger, T.]]
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[[Category: Kwon HJ]]
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[[Category: Kwon, H J.]]
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[[Category: Landy A]]
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[[Category: Landy, A.]]
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[[Category: Nunes-Duby SE]]
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[[Category: Nunes-Duby, S E.]]
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[[Category: protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:25:55 2008''
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Current revision

Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA

PDB ID 1p7d

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