1p8t

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[[Image:1p8t.gif|left|200px]]<br /><applet load="1p8t" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p8t, resolution 3.2&Aring;" />
 
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'''Crystal structure of Nogo-66 Receptor'''<br />
 
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==Overview==
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==Crystal structure of Nogo-66 Receptor==
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The myelin-derived proteins Nogo, MAG and OMgp limit axonal regeneration after injury of the spinal cord and brain. These cell-surface proteins signal through multi-subunit neuronal receptors that contain a common ligand-binding glycosylphosphatidylinositol-anchored subunit termed the Nogo-66 receptor (NgR). By deletion analysis, we show that the binding of soluble fragments of Nogo, MAG and NgR to cell-surface NgR requires the entire leucine-rich repeat (LRR) region of NgR, but not other portions of the protein. Despite sharing extensive sequence similarity with NgR, two related proteins, NgR2 and NgR3, which we have identified, do not bind Nogo, MAG, OMgp or NgR. To investigate NgR specificity and multi-ligand binding, we determined the crystal structure of the biologically active ligand-binding soluble ectodomain of NgR. The molecule is banana shaped with elongation and curvature arising from eight LRRs flanked by an N-terminal cap and a small C-terminal subdomain. The NgR structure analysis, as well as a comparison of NgR surface residues not conserved in NgR2 and NgR3, identifies potential protein interaction sites important in the assembly of a functional signaling complex.
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<StructureSection load='1p8t' size='340' side='right'caption='[[1p8t]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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==Disease==
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<table><tr><td colspan='2'>[[1p8t]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P8T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P8T FirstGlance]. <br>
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Known diseases associated with this structure: Schizophrenia, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=605566 605566]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p8t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p8t OCA], [https://pdbe.org/1p8t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p8t RCSB], [https://www.ebi.ac.uk/pdbsum/1p8t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p8t ProSAT]</span></td></tr>
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1P8T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=NDG:'>NDG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P8T OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/RTN4R_HUMAN RTN4R_HUMAN] Receptor for RTN4, OMG and MAG. Mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult central nervous system. Acts in conjunction with RTN4 and LIGO1 in regulating neuronal precursor cell motility during cortical development (By similarity).<ref>PMID:12037567</ref> <ref>PMID:14966521</ref>
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Structure and axon outgrowth inhibitor binding of the Nogo-66 receptor and related proteins., Barton WA, Liu BP, Tzvetkova D, Jeffrey PD, Fournier AE, Sah D, Cate R, Strittmatter SM, Nikolov DB, EMBO J. 2003 Jul 1;22(13):3291-302. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12839991 12839991]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p8/1p8t_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p8t ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Barton, W A.]]
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[[Category: Barton WA]]
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[[Category: Cate, R.]]
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[[Category: Cate R]]
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[[Category: Fournier, A E.]]
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[[Category: Fournier AE]]
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[[Category: Jeffrey, P D.]]
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[[Category: Jeffrey PD]]
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[[Category: Liu, B P.]]
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[[Category: Liu BP]]
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[[Category: Nikolov, D B.]]
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[[Category: Nikolov DB]]
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[[Category: Sah, D.]]
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[[Category: Sah D]]
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[[Category: Strittmatter, S M.]]
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[[Category: Strittmatter SM]]
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[[Category: Tzvetkova, D.]]
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[[Category: Tzvetkova D]]
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[[Category: NAG]]
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[[Category: NDG]]
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[[Category: ngr]]
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[[Category: nogo-66]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:22 2008''
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Current revision

Crystal structure of Nogo-66 Receptor

PDB ID 1p8t

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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