1p90

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[[Image:1p90.jpg|left|200px]]<br /><applet load="1p90" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p90, resolution 1.8&Aring;" />
 
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'''The Three-dimensional Structure of the Core Domain of NafY from Azotobacter vinelandii determined at 1.8 resolution'''<br />
 
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==Overview==
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==The Three-dimensional Structure of the Core Domain of NafY from Azotobacter vinelandii determined at 1.8 resolution==
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The Azotobacter vinelandii NafY protein (nitrogenase accessory factor Y) is able to bind either to the iron molybdenum cofactor (FeMo-co) or to apodinitrogenase and is believed to facilitate the transfer of FeMo-co into apodinitrogenase. The NafY protein has two domains: an N-terminal domain (residues Met1-Leu98) and a C-terminal domain (residues Glu99-Ser232), referred here to as the "core domain." The core domain of NafY is shown here to be capable of binding the FeMo cofactor of nitrogenase but unable to bind to apodinitrogenase in the absence of the first domain. The three-dimensional molecular structure of the core domain of NafY has been solved to 1.8-A resolution, revealing that the protein consists of a mixed five-stranded beta-sheet flanked by five alpha-helices that belongs to the ribonuclease H superfamily. As such, this represents a new fold capable of binding FeMo-co, where the only previous example was that seen in dinitrogenase.
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<StructureSection load='1p90' size='340' side='right'caption='[[1p90]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1p90]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P90 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P90 FirstGlance]. <br>
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1P90 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Azotobacter_vinelandii Azotobacter vinelandii] with <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P90 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p90 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p90 OCA], [https://pdbe.org/1p90 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p90 RCSB], [https://www.ebi.ac.uk/pdbsum/1p90 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p90 ProSAT]</span></td></tr>
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The three-dimensional structure of the core domain of Naf Y from Azotobacter vinelandii determined at 1.8-A resolution., Dyer DH, Rubio LM, Thoden JB, Holden HM, Ludden PW, Rayment I, J Biol Chem. 2003 Aug 22;278(34):32150-6. Epub 2003 May 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12754195 12754195]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9F5X9_AZOVI Q9F5X9_AZOVI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p90_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p90 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Azotobacter vinelandii]]
[[Category: Azotobacter vinelandii]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dyer, D H.]]
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[[Category: Dyer DH]]
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[[Category: Holden, H M.]]
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[[Category: Holden HM]]
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[[Category: Ludden, P W.]]
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[[Category: Ludden PW]]
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[[Category: Rayment, I.]]
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[[Category: Rayment I]]
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[[Category: Rubio, L M.]]
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[[Category: Rubio LM]]
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[[Category: Thoden, J B.]]
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[[Category: Thoden JB]]
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[[Category: EDO]]
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[[Category: ribonuclease h motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:26 2008''
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Current revision

The Three-dimensional Structure of the Core Domain of NafY from Azotobacter vinelandii determined at 1.8 resolution

PDB ID 1p90

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