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1p9r

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[[Image:1p9r.jpg|left|200px]]<br /><applet load="1p9r" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1p9r, resolution 2.50&Aring;" />
 
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'''Crystal Structure of Vibrio cholerae putative NTPase EpsE'''<br />
 
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==Overview==
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==Crystal Structure of Vibrio cholerae putative NTPase EpsE==
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Type II secretion systems consist of an assembly of 12-15 Gsp proteins responsible for transporting a variety of virulence factors across the outer membrane in several pathogenic bacteria. In Vibrio cholerae, the major virulence factor cholera toxin is secreted by the Eps Type II secretion apparatus consisting of 14 Eps proteins. One of these, EpsE, is a cytoplasmic putative NTPase essential for the functioning of the Eps system and member of the GspE subfamily of Type II secretion ATPases. The crystal structure of a truncated form of EpsE in nucleotide-liganded and unliganded state has been determined, and reveals a two-domain architecture with the four characteristic sequence "boxes" of the GspE subfamily clustering around the nucleotide-binding site of the C-domain. This domain contains two C-terminal subdomains not reported before in this superfamily of NTPases. One of these subdomains contains a four-cysteine motif that appears to be involved in metal binding as revealed by anomalous difference density. The EpsE subunits form a right-handed helical arrangement in the crystal with extensive and conserved contacts between the C and N domains of neighboring subunits. Combining the most conserved interface with the quaternary structure of the C domain in a distant homolog, a hexameric model for EpsE is proposed which may reflect the assembly of this critical protein in the Type II secretion system. The nucleotide ligand contacts both domains in this model. The N2-domain-containing surface of the hexamer appears to be highly conserved in the GspE family and most likely faces the inner membrane interacting with other members of the Eps system.
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<StructureSection load='1p9r' size='340' side='right'caption='[[1p9r]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1p9r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P9R FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p9r OCA], [https://pdbe.org/1p9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p9r RCSB], [https://www.ebi.ac.uk/pdbsum/1p9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p9r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GSPE_VIBCH GSPE_VIBCH] Involved in a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of proteins. Required for secretion of cholera toxin through the outer membrane.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p9/1p9r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p9r ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1P9R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P9R OCA].
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*[[General secretion pathway protein 3D structures|General secretion pathway protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the extracellular protein secretion NTPase EpsE of Vibrio cholerae., Robien MA, Krumm BE, Sandkvist M, Hol WG, J Mol Biol. 2003 Oct 24;333(3):657-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14556751 14556751]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Hol, W G.J.]]
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[[Category: Hol WGJ]]
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[[Category: Krumm, B E.]]
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[[Category: Krumm BE]]
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[[Category: Robien, M A.]]
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[[Category: Robien MA]]
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[[Category: Sandkvist, M.]]
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[[Category: Sandkvist M]]
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[[Category: CL]]
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[[Category: ZN]]
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[[Category: bacterial type ii secretion system cytoplasmic protein - gspe]]
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[[Category: metalloprotein (metal-cys4 site)]]
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[[Category: putative atpase/ atp binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:40 2008''
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Current revision

Crystal Structure of Vibrio cholerae putative NTPase EpsE

PDB ID 1p9r

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