1par

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:06, 14 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1par.gif|left|200px]]<br /><applet load="1par" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1par, resolution 2.600&Aring;" />
 
-
'''DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE'''<br />
 
-
==Overview==
+
==DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE==
-
Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.
+
<StructureSection load='1par' size='340' side='right'caption='[[1par]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[1par]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PAR FirstGlance]. <br>
-
1PAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_phage_py54 Yersinia phage py54]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
-
 
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [https://pdbe.org/1par PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1par RCSB], [https://www.ebi.ac.uk/pdbsum/1par PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1par ProSAT]</span></td></tr>
-
==Reference==
+
</table>
-
DNA recognition by beta-sheets in the Arc repressor-operator crystal structure., Raumann BE, Rould MA, Pabo CO, Sauer RT, Nature. 1994 Feb 24;367(6465):754-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8107872 8107872]
+
== Function ==
-
[[Category: Single protein]]
+
[https://www.uniprot.org/uniprot/RARC_BPP22 RARC_BPP22] This protein acts as a transcriptional repressor of its own gene arc and of gene ant.
-
[[Category: Yersinia phage py54]]
+
__TOC__
-
[[Category: Pabo, C O.]]
+
</StructureSection>
-
[[Category: Raumann, B E.]]
+
[[Category: Large Structures]]
-
[[Category: Rould, M A.]]
+
[[Category: Salmonella virus P22]]
-
[[Category: Sauer, R T.]]
+
[[Category: Pabo CO]]
-
[[Category: double helix]]
+
[[Category: Raumann BE]]
-
[[Category: protein-dna complex]]
+
[[Category: Rould MA]]
-
 
+
[[Category: Sauer RT]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:26:54 2008''
+

Current revision

DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE

PDB ID 1par

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1par"
Personal tools