1bag

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[[Image:1bag.gif|left|200px]]<br />
 
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<applet load="1bag" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bag, resolution 2.5&Aring;" />
 
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'''ALPHA-AMYLASE FROM BACILLUS SUBTILIS COMPLEXED WITH MALTOPENTAOSE'''<br />
 
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==Overview==
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==ALPHA-AMYLASE FROM BACILLUS SUBTILIS COMPLEXED WITH MALTOPENTAOSE==
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The X-ray crystal structure of a catalytic-site mutant EQ208, [Glu208--&gt;Gln] of alpha-amylase from Bacillus subtilis cocrystallized with, maltopentaose (G5) and acarbose has been determined by multiple, isomorphous replacement at 2.5 A resolution. Restrained crystallographic, refinement has resulted in an R-factor of 19.8% in the 7.0 to 2.5 A, resolution range. EQ208 consists of three domains containing a, (beta/alpha)8-barrel as observed in other alpha-amylases. Clear connected, density corresponding to a pentasaccharide was observed, which was, considered as the G5 molecule based on the high affinity of EQ208 for G5, that could replace pre-bound acarbose or a possible transglycosylation, product of acarbose. The conformation around the third, alpha-(1,4)-glucosidic bond makes a sharp ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9514750 (full description)]]
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<StructureSection load='1bag' size='340' side='right'caption='[[1bag]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bag]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BAG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bag OCA], [https://pdbe.org/1bag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bag RCSB], [https://www.ebi.ac.uk/pdbsum/1bag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bag ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMY_BACSU AMY_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ba/1bag_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bag ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BAG is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]]. Structure known Active Sites: CA1, CA2 and CA3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BAG OCA]].
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*[[Amylase 3D structures|Amylase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of a catalytic-site mutant alpha-amylase from Bacillus subtilis complexed with maltopentaose., Fujimoto Z, Takase K, Doui N, Momma M, Matsumoto T, Mizuno H, J Mol Biol. 1998 Mar 27;277(2):393-407. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9514750 9514750]
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[[Category: Alpha-amylase]]
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[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Doui, N.]]
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[[Category: Doui N]]
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[[Category: Fujimoto, Z.]]
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[[Category: Fujimoto Z]]
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[[Category: Mizuno, H.]]
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[[Category: Mizuno H]]
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[[Category: Takase, K.]]
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[[Category: Takase K]]
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[[Category: CA]]
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[[Category: alpha-amylase]]
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[[Category: bacillus subtilis]]
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[[Category: catalytic-site mutant]]
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[[Category: maltopentaose]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:53:39 2007''
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Current revision

ALPHA-AMYLASE FROM BACILLUS SUBTILIS COMPLEXED WITH MALTOPENTAOSE

PDB ID 1bag

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