1pis

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (05:56, 17 April 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1pis.gif|left|200px]]<br /><applet load="1pis" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1pis" />
 
-
'''SOLUTION STRUCTURE OF PORCINE PANCREATIC PHOSPHOLIPASE A2'''<br />
 
-
==Overview==
+
==SOLUTION STRUCTURE OF PORCINE PANCREATIC PHOSPHOLIPASE A2==
-
The lipolytic enzyme phospholipase A2 (PLA2) is involved in the degradation of high-molecular weight phospholipid aggregates in vivo. The enzyme has very high catalytic activities on aggregated substrates compared with monomeric substrates, a phenomenon called interfacial activation. Crystal structures of PLA2s in the absence and presence of inhibitors are identical, from which it has been concluded that enzymatic conformational changes do not play a role in the mechanism of interfacial activation. The high-resolution NMR structure of porcine pancreatic PLA2 free in solution was determined with heteronuclear multidimensional NMR methodology using doubly labeled 13C, 15N-labeled protein. The solution structure of PLA2 shows important deviations from the crystal structure. In the NMR structure the Ala1 alpha-amino group is disordered and the hydrogen bonding network involving the N-terminus and the active site is incomplete. The disorder observed for the N-terminal region of PLA2 in the solution structure could be related to the low activity of the enzyme towards monomeric substrates. The NMR structure of PLA2 suggests, in contrast to the crystallographic work, that conformational changes do play a role in the interfacial activation of this enzyme.
+
<StructureSection load='1pis' size='340' side='right'caption='[[1pis]]' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1pis]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PIS FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pis OCA], [https://pdbe.org/1pis PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pis RCSB], [https://www.ebi.ac.uk/pdbsum/1pis PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pis ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PA21B_PIG PA21B_PIG] PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/1pis_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pis ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1PIS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIS OCA].
+
*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Solution structure of porcine pancreatic phospholipase A2., van den Berg B, Tessari M, de Haas GH, Verheij HM, Boelens R, Kaptein R, EMBO J. 1995 Sep 1;14(17):4123-31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7556053 7556053]
+
[[Category: Large Structures]]
-
[[Category: Phospholipase A(2)]]
+
-
[[Category: Single protein]]
+
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
-
[[Category: Berg, B D.Van Den.]]
+
[[Category: Boelens R]]
-
[[Category: Boelens, R.]]
+
[[Category: De Haas GH]]
-
[[Category: Haas, G H.De.]]
+
[[Category: Kaptein R]]
-
[[Category: Kaptein, R.]]
+
[[Category: Tessari M]]
-
[[Category: Tessari, M.]]
+
[[Category: Van Den Berg BD]]
-
[[Category: Verheij, H M.]]
+
[[Category: Verheij HM]]
-
[[Category: CA]]
+
-
[[Category: phosphatide-2-acyl-hydrolase]]
+
-
[[Category: phospholipase a2]]
+
-
[[Category: pla2]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:12 2008''
+

Current revision

SOLUTION STRUCTURE OF PORCINE PANCREATIC PHOSPHOLIPASE A2

PDB ID 1pis

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1pis"
Personal tools