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1pjq

From Proteopedia

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[[Image:1pjq.gif|left|200px]]<br /><applet load="1pjq" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1pjq, resolution 2.21&Aring;" />
 
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'''Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis'''<br />
 
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==Overview==
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==Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis==
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Sulfur metabolism depends on the iron-containing porphinoid siroheme. In Salmonella enterica, the S-adenosyl-L-methionine (SAM)-dependent bismethyltransferase, dehydrogenase and ferrochelatase, CysG, synthesizes siroheme from uroporphyrinogen III (uro'gen III). The reactions mediated by CysG encompass two branchpoint intermediates in tetrapyrrole biosynthesis, diverting flux first from protoporphyrin IX biosynthesis and then from cobalamin (vitamin B(12)) biosynthesis. We determined the first structure of this multifunctional siroheme synthase by X-ray crystallography. CysG is a homodimeric gene fusion product containing two structurally independent modules: a bismethyltransferase and a dual-function dehydrogenase-chelatase. The methyltransferase active site is a deep groove with a hydrophobic patch surrounded by hydrogen bond donors. This asymmetric arrangement of amino acids may be important in directing substrate binding. Notably, our structure shows that CysG is a phosphoprotein. From mutational analysis of the post-translationally modified serine, we suggest a conserved role for phosphorylation in inhibiting dehydrogenase activity and modulating metabolic flux between siroheme and cobalamin pathways.
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<StructureSection load='1pjq' size='340' side='right'caption='[[1pjq]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1pjq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PJQ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pjq OCA], [https://pdbe.org/1pjq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pjq RCSB], [https://www.ebi.ac.uk/pdbsum/1pjq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pjq ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYSG_SALTY CYSG_SALTY] Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.<ref>PMID:14595395</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pj/1pjq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pjq ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1PJQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with <scene name='pdbligand=ACT:'>ACT</scene>, <scene name='pdbligand=PGE:'>PGE</scene> and <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJQ OCA].
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*[[Siroheme synthase|Siroheme synthase]]
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== References ==
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==Reference==
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<references/>
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CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis., Stroupe ME, Leech HK, Daniels DS, Warren MJ, Getzoff ED, Nat Struct Biol. 2003 Dec;10(12):1064-73. Epub 2003 Nov 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14595395 14595395]
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__TOC__
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[[Category: Salmonella typhimurium]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Daniels, D S.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Getzoff, E D.]]
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[[Category: Daniels DS]]
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[[Category: Leech, H K.]]
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[[Category: Getzoff ED]]
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[[Category: Stroupe, M E.]]
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[[Category: Leech HK]]
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[[Category: Warren, M J.]]
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[[Category: Stroupe ME]]
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[[Category: ACT]]
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[[Category: Warren MJ]]
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[[Category: PGE]]
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[[Category: SAH]]
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[[Category: nad]]
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[[Category: nucleotide binding motif]]
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[[Category: phosphoserine]]
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[[Category: rossman fold]]
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[[Category: sam]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:30 2008''
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Current revision

Structure and function of CysG, the multifunctional methyltransferase/dehydrogenase/ferrochelatase for siroheme synthesis

PDB ID 1pjq

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